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PMID: 2722737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Thermus thermophilus 16S rRNA is transcribed from an isolated transcription unit.

Journal of bacteriology ·Vol. 171 ·No. 6 ·1989-06-00 ·Pages 2933-41

Hartmann RK, Erdmann VA

Abstract

A cloned 16S rRNA gene from the extreme thermophilic eubacterium Thermus thermophilus HB8 was used to characterize the in vivo expression of the 16S rRNA genes in this organism by nuclease S1 mapping. The gene represents an isolated transcription unit encoding solely 16S rRNA. Under exponential growth conditions, transcription was initiated at a single promoter, which represents the structural equivalent of Escherichia coli rrn P2 promoters. The promoter-leader region was very similar to the E. coli rrn P2 promoter-leader segment that is responsible for antitermination. The T. thermophilus leader region was approximately 85 nucleotides shorter than its E. coli P2 counterpart. Potential processing intermediates were correlated with a proposed secondary structure of T. thermophilus pre-16S rRNA.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Bacterial/genetics DNA, Ribosomal/genetics Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic RNA Processing, Post-Transcriptional RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics,ultrastructure Regulatory Sequences, Nucleic Acid Thermus/genetics Transcription, Genetic
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hartmann R K
Institut für Biochemie, Freie Universität Berlin, Federal Republic of Germany.
Erdmann V A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.6.2933-2941.1989
Published
1989-06-00
Pages
2933-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209997
Subset
IM
Databases
GENBANK
M26923, M26924
External Links
PubMed source DOI: 10.1128/jb.171.6.2933-2941.1989 PMC Full Text View journal details
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