Polypeptide hormone binding sites in vivo: initial localization of 125I-labeled insulin to hepatocyte plasmalemma as visualized by electron microscope radioautography.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 5051-5

Bergeron JJ, Levine G, Sikstrom R, O'Shaughnessy D, Kopriwa B, Nadler NJ, Posner BI

Abstract

The rationale of the specific-binding assay was applied to the detection of the liver insulin receptor in vivo. Quantitative electron microscope radioautography indicated that, 3 min after an intraportal injection, 125I-insulin was exclusively located to the hepatocyte plasmalemma.

MeSH Terms

Animals Autoradiography Cell Membrane/metabolism,ultrastructure Iodine Radioisotopes Liver/metabolism,ultrastructure Male Microscopy, Electron Rats Receptor, Insulin/analysis

Chemicals

Iodine Radioisotopes Receptor, Insulin

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Bergeron J J

Levine G

Sikstrom R

O'Shaughnessy D

Kopriwa B

Nadler N J

Posner B I

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.11.5051

Published

1977-11-00

Pages

5051-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432096

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.11.5051 PMC Full Text View journal details

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Polypeptide hormone binding sites in vivo: initial localization of 125I-labeled insulin to hepatocyte plasmalemma as visualized by electron microscope radioautography.

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