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PMID: 2690010 Published · ppublish English Comparative Study Journal Article

Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.

Nucleic acids research ·Vol. 17 ·No. 23 ·1989-12-11 ·Pages 9823-32

Klimasauskas S, Timinskas A, Menkevicius S, Butkienè D, Butkus V, Janulaitis A

Abstract

The sequences coding for DNA[cytosine-N4]methyltransferases MvaI (from Micrococcus varians RFL19) and Cfr9I (from Citrobacter freundii RFL9) have been determined. The predicted methylases are proteins of 454 and 300 amino acids, respectively. Primary structure comparison of M.Cfr9I and another m4C-forming methylase, M.Pvu II, revealed extended regions of homology. The sequence comparison of the three DNA[cytosine-N4]-methylases using originally developed software revealed two conserved patterns, DPF-GSGT and TSPPY, which were found similar also to those of adenine and DNA[cytosine-C5]-methylases. These data provided a basis for global alignment and classification of DNA-methylase sequences. Structural considerations led us to suggest that the first region could be the binding site of AdoMet, while the second is thought to be directly involved in the modification of the exocyclic amino group.

MeSH Terms
Amino Acid Sequence Base Sequence Citrobacter/enzymology,genetics DNA (Cytosine-5-)-Methyltransferases/genetics DNA, Bacterial/genetics DNA-Cytosine Methylases/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Micrococcus/enzymology,genetics Molecular Sequence Data Sequence Homology, Nucleic Acid Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics
Chemicals
DNA, Bacterial DNA modification methylase EcoRII DNA modification methylase XcyI DNA-Cytosine Methylases DNA (Cytosine-5-)-Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Klimasauskas S
Institute of Applied Enzymology, Vilnius, Lithuania, USSR.
Timinskas A
Menkevicius S
Butkienè D
Butkus V
Janulaitis A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.23.9823
Published
1989-12-11
Pages
9823-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335216
Subset
IM
Databases
GENBANK
X16985, X17022
External Links
PubMed source DOI: 10.1093/nar/17.23.9823 PMC Full Text View journal details
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