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PMID: 2685557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 10 ·1989-10-00 ·Pages 4571-5

Song JM, Picologlou S, Grant CM, Firoozan M, Tuite MF, Liebman S

Abstract

Changes in the dosage of genes encoding elongation factor EF-1 alpha were shown to cause parallel changes in the misreading of nonsense codons. Higher amounts of EF-1 alpha were correlated with increased nonsense suppression, suggesting that the level of EF-1 alpha is critically involved in translational fidelity.

MeSH Terms
Cloning, Molecular Gene Amplification/physiology Paromomycin/pharmacology Peptide Elongation Factor 1 Peptide Elongation Factors/biosynthesis,genetics Protein Biosynthesis/physiology Restriction Mapping Saccharomyces cerevisiae/genetics Suppression, Genetic/physiology
Chemicals
Peptide Elongation Factor 1 Peptide Elongation Factors Paromomycin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Song J M
Department of Biological Sciences, University of Illinois, Chicago 60680.
Picologlou S
Grant C M
Firoozan M
Tuite M F
Liebman S
References (34)
34 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae.
    Genetics. 1988 Dec;120(4):923-34 PMID: 3066688
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. Isolation and characterization of amber suppressors in yeast.
    Genetics. 1976 Feb;82(2):251-72 PMID: 177333
  5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  6. The role of guanosine 5'-triphosphate in polypeptide chain elongation.
    Biochim Biophys Acta. 1978 Sep 21;505(1):95-127 PMID: 361078
  7. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  8. An antisuppressor that acts on omnipotent suppressors in yeast.
    Genetics. 1980 May;95(1):49-61 PMID: 7000618
  9. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  10. Structural analysis and sequence organization of yeast centromeres.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1175-85 PMID: 6305576
  11. Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.
    Gene. 1983 Sep;24(1):1-14 PMID: 6313478
  12. The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.
    J Bacteriol. 1983 Nov;156(2):625-35 PMID: 6313610
  13. Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.
    Mol Gen Genet. 1984;193(3):557-60 PMID: 6323928
  14. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2431-4 PMID: 6326126
  15. Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.
    EMBO J. 1984 Aug;3(8):1825-30 PMID: 6383821
  16. EFTu provides an internal kinetic standard for translational accuracy.
    Trends Biochem Sci. 1988 Mar;13(3):91-3 PMID: 3072707
  17. Mistranslation induces the heat-shock response in the yeast Saccharomyces cerevisiae.
    Mol Microbiol. 1989 Feb;3(2):215-20 PMID: 2548059
  18. Host-plasmid interactions in Saccharomyces cerevisiae: effect of host ploidy on plasmid stability and copy number.
    J Gen Microbiol. 1989 Apr;135(4):1037-45 PMID: 2689592
  19. Phosphorylation of elongation factor 1 in polyribosome fraction of rabbit reticulocytes.
    FEBS Lett. 1984 Oct 29;176(2):401-5 PMID: 6489525
  20. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.
    EMBO J. 1984 Dec 20;3(13):3311-5 PMID: 6396088
  21. Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.
    J Biol Chem. 1985 Mar 10;260(5):3090-6 PMID: 2982849
  22. Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product.
    Mol Cell Biol. 1985 Apr;5(4):816-22 PMID: 3887137
  23. Mutants of the elongation factor EF-Tu, a new class of nonsense suppressors.
    EMBO J. 1985 Apr;4(4):1049-52 PMID: 3926487
  24. Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene.
    Nucleic Acids Res. 1986 Jul 11;14(13):5187-97 PMID: 3526282
  25. Mutant EF-Tu increases missense error in vitro.
    Mol Gen Genet. 1986 Oct;205(1):186-8 PMID: 3540529
  26. Structure of the two genes coding for polypeptide chain elongation factor 1 alpha (EF-1 alpha) from Saccharomyces cerevisiae.
    Gene. 1986;45(3):265-73 PMID: 3026912
  27. Mutant forms of tufA and tufB independently suppress nonsense mutations.
    J Mol Biol. 1987 Oct 20;197(4):611-5 PMID: 3323524
  28. Mutants of elongation factor Tu promote ribosomal frameshifting and nonsense readthrough.
    EMBO J. 1987 Dec 20;6(13):4235-9 PMID: 3327691
  29. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.
    Gene. 1987;60(2-3):237-43 PMID: 3327750
  30. SUF12 suppressor protein of yeast. A fusion protein related to the EF-1 family of elongation factors.
    J Mol Biol. 1988 Feb 20;199(4):559-73 PMID: 3280807
  31. Either one of the two yeast EF-1 alpha genes is required for cell viability.
    Curr Genet. 1985;9(8):693-7 PMID: 3916735
  32. Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation.
    Nature. 1988 Jul 14;334(6178):170-3 PMID: 3386756
  33. Nucleotide sequence of the SUP2 (SUP35) gene of Saccharomyces cerevisiae.
    Gene. 1988 Jun 15;66(1):45-54 PMID: 3047009
  34. Suppressors in yeast.
    Curr Top Microbiol Immunol. 1974;64(0):1-47 PMID: 4602646
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.10.4571-4575.1989
Published
1989-10-00
Pages
4571-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362547
Subset
IM
Grants
NIGMS NIH HHS · GM 24189 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.10.4571-4575.1989 PMC Full Text View journal details
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