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PMID: 2685354 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteolytic origin of the 150-kilodalton protein encoded by turnip yellow mosaic virus genomic RNA.

Journal of virology ·Vol. 63 ·No. 12 ·1989-12-00 ·Pages 5153-8

Morch MD, Drugeon G, Szafranski P, Haenni AL

Abstract

Turnip yellow mosaic virus genomic RNA codes in vitro for two overlapping proteins, 150-kilodalton (150K protein) and 206-kilodalton (206K protein) proteins. The proteolytic maturation known to affect the 206K protein has been further characterized by in vitro translation assays in a reticulocyte lysate or wheat germ extract. Cleavage is inhibited at 37 degrees C and restored when the temperature is shifted to 30 or 25 degrees C. Temperature shift experiments are used here to demonstrate that the 150K protein and the previously characterized 78K protein are the two fragments resulting from a primary cleavage phenomenon that affects the 206K protein in a cotranslational manner under usual translation conditions. This processing is prevented by several cysteine and serine proteinase inhibitors.

MeSH Terms
Gene Expression Genes, Viral Kinetics Molecular Weight Mosaic Viruses/genetics Peptide Hydrolases/metabolism Protease Inhibitors/pharmacology Protein Biosynthesis RNA, Viral/genetics Thermodynamics Viral Proteins/genetics Viral Structural Proteins/genetics
Chemicals
Protease Inhibitors RNA, Viral Viral Proteins Viral Structural Proteins Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morch M D
Institut Jacques Monod, Paris, France.
Drugeon G
Szafranski P
Haenni A L
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.12.5153-5158.1989
Published
1989-12-00
Pages
5153-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC251178
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.12.5153-5158.1989 PMC Full Text View journal details
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