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PMID: 2677028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Presence of a protein immunologically related to lamin B in the postsynaptic membrane of Torpedo marmorata electrocyte.

The Journal of cell biology ·Vol. 109 ·No. 4 Pt 1 ·1989-10-00 ·Pages 1745-52

Cartaud A, Courvalin JC, Ludosky MA, Cartaud J

Abstract

The Torpedo electrocyte is a flattened syncytium derived from skeletal muscle, characterized by two functionally distinct plasma membrane domains. The electrocyte is filled up with a transversal network of intermediate filaments (IF) of desmin which contact in an end-on fashion both sides of the cell. In this work, we show that polyclonal antibodies specific for lamin B recognizes a component of the plasma membrane of Torpedo electrocyte. This protein which thus shares epitopes with lamin B has a relative molecular mass of 54 kD, an acidic IP of 5.4. It is localized exclusively on the cytoplasmic side of the innervated membrane of the electrocyte at sites of IF-membrane contacts. Since our previous work showed that the noninnervated membrane contains ankyrin (Kordeli, E., J. Cartaud, H. O. Nghiêm, L. A. Pradel, C. Dubreuil, D. Paulin, and J.-P. Changeux. 1986. J. Cell Biol. 102:748-761), the present results suggest that desmin filaments may be anchored via the 54-kD protein to the innervated membrane and via ankyrin to the noninnervated membrane. These findings would represent an extension of the model proposed by Georgatos and Blobel (Georgatos, S. D., and G. Blobel. 1987a. J. Cell Biol. 105:105-115) in which type III intermediate size filaments are vectorially inserted to plasma and nuclear membranes by ankyrin and lamin B, respectively.

MeSH Terms
Animals Electric Organ/analysis,cytology,ultrastructure Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Immunoblotting Lamin Type B Lamins Molecular Weight Muscles/analysis Nuclear Proteins/analysis,immunology Rats Synaptic Membranes/analysis,ultrastructure Torpedo
Chemicals
Lamin Type B Lamins Nuclear Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cartaud A
Microscopie Electronique et Biologie Cellulaire des Membranes, Institut J. Monod, Centre National de la Recherche Scientique, Université Paris VII, France.
Courvalin J C
Ludosky M A
Cartaud J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.4.1745
Published
1989-10-00
Pages
1745-52
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115797
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.109.4.1745 PMC Full Text View journal details
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