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PMID: 2674944 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for two pathways of meiotic intrachromosomal recombination in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 7072-6

Gottlieb S, Wagstaff J, Esposito RE

Abstract

This study shows that RAD50, a yeast DNA repair gene required for meiotic interchromosomal exchange between homologs, also is required for meiotic intrachromosomal recombination. However, only intrachromosomal events in nonribosomal DNA are dependent on RAD50; those in ribosomal DNA (rRNA-encoding DNA) occur in the absence of this gene. Furthermore, nonribosomal DNA sequences retain their RAD50-dependence even when inserted into the ribosomal DNA array. We argue that these data provide evidence for at least two pathways of meiotic intrachromosomal recombination whose activity depends on the specific sequences involved or their structural context in the chromosome. In contrast to its role in meiosis, RAD50 is not required for either inter- or intrachromosomal spontaneous mitotic recombination.

MeSH Terms
Chromosomes/physiology Cloning, Molecular DNA, Fungal/genetics Genes, Fungal Genotype Meiosis Mitosis Phenotype Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics,physiology
Chemicals
DNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gottlieb S
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Wagstaff J
Esposito R E
References (32)
32 references, click to expand
  1. Gene conversion between duplicated genetic elements in yeast.
    Nature. 1981 Jul 23;292(5821):306-11 PMID: 6265790
  2. Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division.
    Genetics. 1980 Nov;96(3):567-88 PMID: 7021311
  3. The genetic control of meiosis.
    Annu Rev Genet. 1976;10:53-134 PMID: 797314
  4. Yeast ribosomal DNA genes are located on chromosome XII.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):410-4 PMID: 370829
  5. Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4997-5001 PMID: 6308665
  6. A genetic study of x-ray sensitive mutants in yeast.
    Mutat Res. 1974 Sep;24(3):281-92 PMID: 4606119
  7. Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5056-60 PMID: 1108011
  8. A new mapping method employing a meiotic rec-mutant of yeast.
    Genetics. 1982 Mar;100(3):387-412 PMID: 6749597
  9. Recombinational substrates designed to study recombination between unique and repetitive sequences in vivo.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6215-9 PMID: 3306671
  10. Genetic map of Saccharomyces cerevisiae.
    Microbiol Rev. 1980 Dec;44(4):519-71 PMID: 7010111
  11. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events.
    Nature. 1983 Feb 24;301(5902):671-4 PMID: 6828150
  12. Cloning and mapping of the RAD50 gene of Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;193(3):525-31 PMID: 6323924
  13. A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA.
    Cell. 1989 Mar 10;56(5):771-6 PMID: 2647300
  14. Meiosis in haploid yeast.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2986-90 PMID: 7045878
  15. Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination.
    Genetics. 1986 Jul;113(3):551-67 PMID: 3015718
  16. Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.
    Nature. 1980 Apr 3;284(5755):426-30 PMID: 6987539
  17. Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes.
    Cell. 1980 Mar;19(3):765-74 PMID: 6988084
  18. Recombinationless meiosis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Oct;1(10):891-901 PMID: 7050657
  19. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  20. Gene conversion, recombination nodules, and the initiation of meiotic synapsis.
    Bioessays. 1987 May;6(5):232-6 PMID: 3606589
  21. Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5091-5 PMID: 337310
  22. The role of the SPO11 gene in meiotic recombination in yeast.
    Genetics. 1985 Jun;110(2):187-216 PMID: 3891509
  23. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.
    Nature. 1981 Mar 5;290(5801):26-9 PMID: 7010180
  24. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
    Cell. 1980 Oct;21(3):627-38 PMID: 7438203
  25. The synaptonemal complex in genetic segregation.
    Annu Rev Genet. 1984;18:331-413 PMID: 6241453
  26. Meiotic recombination between duplicated genetic elements in Saccharomyces cerevisiae.
    Genetics. 1985 Feb;109(2):303-32 PMID: 3882522
  27. The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast.
    Genetics. 1980 Jan;94(1):51-68 PMID: 17248996
  28. Intrachromosomal gene conversion and the maintenance of sequence homogeneity among repeated genes.
    Genetics. 1982 Feb;100(2):315-37 PMID: 7106560
  29. Ring chromosomes and rDNA magnification in Drosophila.
    Genetics. 1984 Dec;108(4):969-83 PMID: 6439601
  30. MAR1-a Regulator of the HMa and HMalpha Loci in SACCHAROMYCES CEREVISIAE.
    Genetics. 1979 Sep;93(1):37-50 PMID: 17248968
  31. Meiotic exchange within and between chromosomes requires a common Rec function in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Dec;5(12):3532-44 PMID: 3915779
  32. Genetic control of diploid recovery after gamma-irradiation in the yeast Saccharomyces cerevisiae.
    Mutat Res. 1980 Dec;73(2):251-65 PMID: 7007877
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.7072
Published
1989-09-00
Pages
7072-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297996
Subset
IM
Grants
NIGMS NIH HHS · GM07197 · United States
NIGMS NIH HHS · GM29182 · United States
NICHD NIH HHS · HD19252 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.18.7072 PMC Full Text View journal details
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