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PMID: 2671659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 217 ·No. 2-3 ·1989-06-00 ·Pages 355-61

Schulz R, Steinmüller K, Klaas M, Forreiter C, Rasmussen S, Hiller C, Apel K

Abstract

The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Escherichia coli/genetics Molecular Sequence Data Oxidoreductases/genetics Oxidoreductases Acting on CH-CH Group Donors Plants/enzymology,genetics
Chemicals
DNA Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors protochlorophyllide reductase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schulz R
Botanisches Institut, Christian-Albrechts-Universität Kiel, Federal Republic of Germany.
Steinmüller K
Klaas M
Forreiter C
Rasmussen S
Hiller C
Apel K
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02464904
Published
1989-06-00
Pages
355-61
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X15869
External Links
PubMed source DOI: 10.1007/BF02464904
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