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PMID: 266707 Published · ppublish English Journal Article

Identification of Rauscher murine leukemia virus-specific mRNAs for the synthesis of gag- and env-gene products.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 5 ·1977-05-00 ·Pages 1855-9

Zaane DV, Gielkens AL, Hesselink WG, Bloemers HP

Abstract

Polyadenylylated mRNA isolated from cells infected with Rauscher murine leukemia virus was fractionated by centrifugation in in a denaturing sucrose gradient into different sizes. Each RNA fraction was injected into oocytes of Xenopus laevis and the virus-specific products were analyzed by immunoprecipitation with polyvalent and monospecific antisera against polypeptides of Rauscher murine leukemia virus, and then by gel electrophoresis and scintillation autoradiography. It was shown that a 35S mRNA species directs the synthesis of a precursor of the internal or group-specific antigens of the virion (the gag-gene products). A 22S mRNA species directs the synthesis of two viral envelope polypeptides and their precursor polypeptide (env-gene products). The results indicate that the gag- and env-related polypeptides of Rauscher murine leukemia virus are synthesized uncoordinately and provide evidence for open and closed cistrons on the virus-specific mRNAs.

MeSH Terms
Antigen-Antibody Reactions Cell-Free System Genes Genes, Viral Molecular Weight Poly A/metabolism Protein Precursors/biosynthesis RNA, Messenger/isolation & purification,metabolism RNA, Viral/isolation & purification,metabolism Rauscher Virus/metabolism Viral Proteins/biosynthesis,immunology
Chemicals
Protein Precursors RNA, Messenger RNA, Viral Viral Proteins Poly A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zaane D V
Gielkens A L
Hesselink W G
Bloemers H P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.5.1855
Published
1977-05-00
Pages
1855-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431029
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.5.1855 PMC Full Text View journal details
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