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PMID: 2664470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 5 ·1989-05-00 ·Pages 2142-52

Smith HE, Mitchell AP

Abstract

Two signals activate meiosis in yeast: starvation and expression of the a1 and alpha 2 products of the mating-type locus. Prior studies suggest that these signals stimulate expression of an activator of meiosis, the IME1 (inducer of meiosis) product. We have cloned a gene, IME2, with properties similar to those of IME1: both genes are required for meiosis, and both RNAs are induced in meiotic cells. Elevated dosage of IME1 or IME2 stimulates the meiotic recombination pathway without starvation; thus, the IME products may be part of the switch that activates meiosis. IME1 was found to be required for IME2 expression, and a multicopy IME2 plasmid permitted meiosis in an ime1 deletion mutant. Accordingly, we propose that the IME1 product stimulates meiosis mainly through activation of IME2 expression.

MeSH Terms
Cloning, Molecular Gene Expression Regulation Genes, Fungal Genes, Mating Type, Fungal Meiosis Saccharomyces cerevisiae/cytology,genetics,physiology Spores, Fungal/genetics Transcription, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith H E
Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Mitchell A P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.5.2142-2152.1989
Published
1989-05-00
Pages
2142-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363008
Subset
IM
Grants
NIGMS NIH HHS · GM39531 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.5.2142-2152.1989 PMC Full Text View journal details
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