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PMID: 2662192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4982-6

Kuang J, Zhao J, Wright DA, Saunders GF, Rao PN

Abstract

MPM-2, a monoclonal antibody specific for cells in mitosis, recognizes a family of proteins that share a common phosphorylated epitope. In this study we have shown that during the maturation of Xenopus laevis oocytes induced by progesterone, phosphorylation of MPM-2 antigens coincided with the appearance of MPF activity. When MPM-2 (0.7-1.4 micrograms per oocyte) was injected into oocytes prior to progesterone stimulation, MPF activity failed to appear and induction of maturation was inhibited as judged by both germinal-vesicle breakdown and white-spot formation. Further, MPM-2 was able to neutralize as well as immunodeplete MPF activity from mitotic HeLa cell and mature oocyte extracts. These results suggest that MPM-2 recognizes either MPF itself or a protein(s) that regulates MPF activity and that the kinase that phosphorylates MPM-2 antigens may be a key component in the regulation of M-phase induction.

MeSH Terms

Animals Antibodies, Monoclonal/isolation & purification Antigen-Antibody Complex Female Growth Substances/isolation & purification,pharmacology HeLa Cells/cytology Humans Kinetics Maturation-Promoting Factor Mitosis Oocytes/cytology,drug effects,physiology Xenopus laevis

Chemicals

Antibodies, Monoclonal Antigen-Antibody Complex Growth Substances Maturation-Promoting Factor

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kuang J

Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.

Zhao J

Wright D A

Saunders G F

Rao P N

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.13.4982

Published

1989-07-00

Pages

4982-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC297540

Subset

Grants

NCI NIH HHS · CA27544 · United States

NCI NIH HHS · CA34783 · United States

External Links

PubMed source DOI: 10.1073/pnas.86.13.4982 PMC Full Text View journal details

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Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.

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