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PMID: 2651900 Published · ppublish English Journal Article

Identification of an upstream activating sequence and an upstream repressible sequence of the pyruvate kinase gene of the yeast Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 2 ·1989-02-00 ·Pages 442-51

Nishizawa M, Araki R, Teranishi Y

Abstract

To clarify carbon source-dependent control of the glycolytic pathway in the yeast Saccharomyces cerevisiae, we have initiated a study of transcriptional regulation of the pyruvate kinase gene (PYK). By deletion analysis of the 5'-noncoding region of the PYK gene, we have identified an upstream activating sequence (UASPYK1) located between 634 and 653 nucleotides upstream of the initiating ATG codon. The promoter activity of the PYK 5'-noncoding region was abolished when the sequence containing the UASPYK1 was deleted from the region. Synthetic UASPYK1 (26mer), in either orientation, was able to restore the transcriptional activity of UAS-depleted mutants when placed upstream of the TATA sequence located at -199 (ATG as +1). While the UASPYK1 was required for basal to intermediate levels of transcriptional activation, a sequence between -714 and -811 was found to be necessary for full activation. On the other hand, a sequence between -344 and -468 was found to be responsible for transcriptional repression of the PYK gene when yeast cells were grown on nonfermentable carbon sources. This upstream repressible sequence also repressed transcription, although to a lesser extent, when glucose was present in the medium. The possible mechanism for carbon source-dependent regulation of PYK expression through these cis-acting regulatory elements is discussed.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Genes, Regulator Genes, Synthetic Molecular Sequence Data Plasmids Pyruvate Kinase/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Messenger Pyruvate Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nishizawa M
Biosciences Laboratory, Mitsubishi Kasei Corporation, Yokohama, Japan.
Araki R
Teranishi Y
References (41)
41 references, click to expand
  1. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  2. Changes in the concentration of cAMP, fructose 2,6-bisphosphate and related metabolites and enzymes in Saccharomyces cerevisiae during growth on glucose.
    Eur J Biochem. 1987 Apr 15;164(2):369-73 PMID: 3032616
  3. The meta cleavage of catechol by Azotobacter species. 4-Oxalocrotonate pathway.
    Eur J Biochem. 1971 Jun 11;20(3):400-13 PMID: 4325686
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. A simplification of the protein assay method of Lowry et al. which is more generally applicable.
    Anal Biochem. 1977 Dec;83(2):346-56 PMID: 603028
  7. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  8. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  9. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation.
    J Bacteriol. 1980 Sep;143(3):1384-94 PMID: 6997270
  10. Molecular cloning of TOL genes xylB and xylE in Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1137-43 PMID: 7009570
  11. The gcr (glycolysis regulation) mutation of Saccharomyces cerevisiae.
    J Biol Chem. 1981 Dec 25;256(24):13074-8 PMID: 7031056
  12. Transcription of the his3 gene region in Saccharomyces cerevisiae.
    J Mol Biol. 1981 Nov 5;152(3):535-52 PMID: 6173489
  13. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  14. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355-9 PMID: 6285379
  15. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  16. Cloning of yeast glycolysis genes by complementation.
    Biochem Biophys Res Commun. 1982 Oct 15;108(3):1107-22 PMID: 6295367
  17. The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae.
    J Biol Chem. 1983 Feb 25;258(4):2193-201 PMID: 6185493
  18. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  19. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1101-5 PMID: 6405380
  20. Use of statistical criteria for screening potential homologies in nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):203-13 PMID: 6694901
  21. Transcription and regulatory signals at the mating type locus in yeast.
    Cell. 1984 Jul;37(3):969-78 PMID: 6378388
  22. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.
    Mol Cell Biol. 1984 Nov;4(11):2467-78 PMID: 6392852
  23. Deletion analysis identifies a region, upstream of the ADH2 gene of Saccharomyces cerevisiae, which is required for ADR1-mediated derepression.
    Mol Cell Biol. 1985 Jul;5(7):1743-9 PMID: 3160930
  24. Transcriptional and post-transcriptional regulation of L-type pyruvate kinase in diabetic rat liver by insulin and dietary fructose.
    J Biol Chem. 1985 Nov 15;260(26):14393-7 PMID: 2414297
  25. Conserved sequence elements upstream of the gene encoding yeast ribosomal protein L25 are involved in transcription activation.
    EMBO J. 1986 May;5(5):1037-40 PMID: 3013611
  26. Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.
    Mol Cell Biol. 1986 Jul;6(7):2287-97 PMID: 3537717
  27. The UAS of the yeast PGK gene contains functionally distinct domains.
    Nucleic Acids Res. 1987 Sep 11;15(17):6855-73 PMID: 3309889
  28. Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences.
    Mol Cell Biol. 1987 Aug;7(8):2753-61 PMID: 3313003
  29. Identification of sequence elements that confer cell-type-specific control of MF alpha 1 expression in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Sep;7(9):3185-93 PMID: 2959859
  30. Translation and stability of an Escherichia coli beta-galactosidase mRNA expressed under the control of pyruvate kinase sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1987 Oct 12;15(19):7963-74 PMID: 2444925
  31. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements.
    Cell. 1987 Dec 4;51(5):721-32 PMID: 3315231
  32. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jan;8(1):210-25 PMID: 3275867
  33. Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8839-43 PMID: 3321057
  34. The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1.
    EMBO J. 1987 Dec 1;6(12):3755-60 PMID: 2828036
  35. Regulation of gluconeogenic enzymes during the cell cycle of Saccharomyces cerevisiae growing in a chemostat.
    J Gen Microbiol. 1987 Sep;133(9):2517-22 PMID: 2834508
  36. Metapyrocatachase: a new catechol-cleaving enzyme.
    J Biol Chem. 1961 Aug;236:2223-8 PMID: 13757654
  37. Glycolytic gene expression in Saccharomyces cerevisiae: nucleotide sequence of GCR1, null mutants, and evidence for expression.
    Mol Cell Biol. 1986 Nov;6(11):3774-84 PMID: 3025612
  38. Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.
    Mol Cell Biol. 1986 Dec;6(12):4335-43 PMID: 3540610
  39. The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Feb;7(2):813-20 PMID: 3547083
  40. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  41. Control of glycolytic enzyme synthesis in yeast by products of the hexokinase reaction.
    J Biol Chem. 1971 Jan 25;246(2):489-99 PMID: 4250748
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.2.442-451.1989
Published
1989-02-00
Pages
442-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362619
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.2.442-451.1989 PMC Full Text View journal details
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