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PMID: 2649129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HLA class I gene expression on human primary tumours and autologous metastases: demonstration of selective losses of HLA antigens on colorectal, gastric and laryngeal carcinomas.

British journal of cancer ·Vol. 59 ·No. 2 ·1989-02-00 ·Pages 221-6

López-Nevot MA, Esteban F, Ferrón A, Gutiérrez J, Oliva MR, Romero C, Huelin C, Ruiz-Cabello F, Garrido F

Abstract

The expression of HLA class I antigens was studied in 99 primary tumour (colorectal, gastric and laryngeal carcinomas) and 57 autologous metastases using immunohistological techniques and monoclonal antibodies against class I monomorphic determinants, HLA-B isotypic determinants and HLA polymorphic determinants. Fourteen per cent of colorectal, 9.6% of gastric and 20% of laryngeal carcinomas completely lacked class I molecules. Selective losses of HLA-B antigens were also detected in 8.8, 3.4 and 5.8% of these tumours respectively. Taking into account complete and selective loss of HLA-B the average alteration in the class I molecules expression totalled 21%. The comparison of class I expression between primary tumours and autologous metastases showed differences in 24% of the patients. These differences consisted mainly in a decrease of class I expression by metastases. Nevertheless, four types of divergence were detected in laryngeal carcinomas, namely: +/-, +/+, -/+, -/-. In addition, a clear correlation between degree of differentiation and class I expression was observed in laryngeal tumours. Finally, when class I gene RFLPs were compared with DNA from 15 tumours and autologous normal mucosa or peripheral lymphocytes, no differences were detected between these samples.

MeSH Terms
Blotting, Southern Colorectal Neoplasms/genetics,immunology Genes, MHC Class I HLA Antigens/analysis HLA-B Antigens/analysis Humans Immunoenzyme Techniques Laryngeal Neoplasms/genetics,immunology Lymphatic Metastasis Stomach Neoplasms/genetics,immunology
Chemicals
HLA Antigens HLA-B Antigens
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
López-Nevot M A
Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada, Spain.
Esteban F
Ferrón A
Gutiérrez J
Oliva M R
Romero C
Huelin C
Ruiz-Cabello F
Garrido F
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1989.45
Published
1989-02-00
Pages
221-6
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2246991
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1989.45 PMC Full Text View journal details
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