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PMID: 2645294 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Processing pathway for protease B of Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 108 ·No. 2 ·1989-02-00 ·Pages 309-25

Moehle CM, Dixon CK, Jones EW

Abstract

The vacuolar protease B of Saccharomyces cerevisiae is a subtilisin-like protease encoded by the PRB1 gene. Antibodies raised against a synthetic peptide and an Escherichia coli-derived PRB1 open reading frame (ORF) protein cross-react with authentic protease B from yeast. By using these antibodies, the posttranslational biosynthetic pathway of protease B has been elucidated. Preproprotease B is a 76-kD unglycosylated precursor that enters the endoplasmic reticulum (ER), where it receives one asparagine-linked (Asn-linked) and an undetermined number of non-Asn-linked carbohydrate side chains. The large glycosylated intermediate is proteolytically processed to a 39-kD form before exiting the ER. In the Golgi complex, the 39-kD form becomes 40 kD, due to elaboration of the Asn-linked side chain. The carboxyterminal end of the 40-kD proprotease B undergoes protease A-mediated processing to a 37-kD intermediate, which in turn is quickly processed to 31-kD mature protease B. The ultimate processing step removes a peptide containing the Asn-linked chain; mature PrB has only non-Asn-linked carbohydrate.

MeSH Terms
Asparagine/metabolism Cytoplasm/enzymology Endoplasmic Reticulum/enzymology Enzyme Precursors/metabolism Escherichia coli/enzymology,genetics Glycosylation Golgi Apparatus/enzymology Immunoblotting Immunosorbent Techniques Mutation Protein Processing, Post-Translational Saccharomyces cerevisiae/enzymology,genetics Serine Endopeptidases/biosynthesis,genetics Vacuoles/enzymology
Chemicals
Enzyme Precursors Asparagine Serine Endopeptidases yeast proteinase B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moehle C M
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
Dixon C K
Jones E W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.2.309
Published
1989-02-00
Pages
309-25
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115423
Subset
IM
Grants
NIDDK NIH HHS · DK18090 · United States
NIGMS NIH HHS · GM29713 · United States
NIGMS NIH HHS · T32GM08067 · United States
External Links
PubMed source DOI: 10.1083/jcb.108.2.309 PMC Full Text View journal details
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