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PMID: 377296 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Septum formation, cell division, and sporulation in mutants of yeast deficient in proteinase B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2395-9

Zubenko GS, Mitchell AP, Jones EW

Abstract

Mutants of Saccharomyces cerevisiae that carry lesions in the structural gene for proteinase B, prb1, have been isolated. Proteinase B was thought to be involved in activation of chitin synthase zymogen to allow septum formation during budding and to be involved in sporulation. The prb1 mutants are able to grow and divide and they form apparently normal septa that contain chitin. Most diploids homozygous for prb1 mutations are unable to complete sporulation.

MeSH Terms
Alleles Cell Division Cell Wall Endopeptidases Genes Hot Temperature Mutation Peptide Hydrolases/genetics,physiology Saccharomyces cerevisiae/cytology,enzymology,genetics Saccharomyces cerevisiae Proteins Spores, Fungal/physiology
Chemicals
Saccharomyces cerevisiae Proteins Endopeptidases PRB1 protein, S cerevisiae Peptide Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zubenko G S
Mitchell A P
Jones E W
References (24)
24 references, click to expand
  1. ACTION OF A SUPER-SUPPRESSOR IN YEAST IN RELATION TO ALLELIC MAPPING AND COMPLEMENTATION.
    Genetics. 1964 Jul;50:109-21 PMID: 14191344
  2. Observations on the cell wall of yeasts; an electron microscope and x-ray diffraction study.
    Antonie Van Leeuwenhoek. 1953;19(1):1-24 PMID: 13041175
  3. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  4. Characterization and genetic mapping of modA. A mutation in the post-translational modification of the glycosidases of Dictyostelium discoideum.
    J Biol Chem. 1978 Jun 25;253(12):4102-6 PMID: 96112
  5. The structural gene for alpha-mannosidase-1 in Dictyostellium discoideum.
    Genetics. 1976 Oct;84(2):159-74 PMID: 1033886
  6. Inactivation of yeast enzymes by proteinase A and B and carboxypeptidase Y from yeast.
    Hoppe Seylers Z Physiol Chem. 1976 May;357(5):735-40 PMID: 184028
  7. Proteinase mutants of Saccharomyces cerevisiae.
    Genetics. 1977 Jan;85(1):23-33 PMID: 320092
  8. Specific inactivation of fructose 1,6-bisphosphatase from Saccharomyces cerevisiae by a yeast protease.
    Eur J Biochem. 1974 May 2;44(1):213-17 PMID: 4369197
  9. Mutations affecting levels of tetrahydrofolate interconversion enzymes in Saccharomyces cerevisiae. II. Map positions on chromosome VII of ade3-41 and ADE15.
    Mol Gen Genet. 1973 Jul 2;123(3):209-18 PMID: 4147244
  10. Activities and cellular localization of yeast proteases and their inhibitors.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1378-83 PMID: 4609372
  11. Compartmentation of the tryptophan-synthase-proteolyzing system in Saccharomyces cerevisiae.
    Eur J Biochem. 1974 Oct 1;48(1):117-7 PMID: 4614972
  12. Yeast chitin synthetase. Separation of the zymogen from its activating factor and recovery of the latter in the vacuole fraction.
    J Biol Chem. 1973 Feb 25;248(4):1451-8 PMID: 4568818
  13. Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.
    J Bacteriol. 1975 Dec;124(3):1604-6 PMID: 372
  14. The activating system of chitin synthetase from Saccharomyces cerevisiae. Purification and properties of the activating factor.
    J Biol Chem. 1976 Jun 10;251(11):3367-74 PMID: 6452
  15. Three yeast proteins that specifically inhibit yeast proteases A, B, and C.
    J Bacteriol. 1975 Jun;122(3):1265-73 PMID: 238943
  16. Protein degradation and proteinases during yeast sporulation.
    Eur J Biochem. 1976 Feb 2;62(1):65-76 PMID: 814003
  17. Timing and function of chitin synthesis in yeast.
    J Bacteriol. 1975 Dec;124(3):1586-93 PMID: 1104591
  18. Chitin synthetase zymogen is attached to the yeast plasma membrane.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3952-5 PMID: 1105572
  19. Chitin and yeast budding. Localization of chitin in yeast bud scars.
    J Biol Chem. 1971 Jan 10;246(1):152-9 PMID: 5541754
  20. The glucan components of the cell wall of baker's yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure.
    Biochem J. 1969 Sep;114(3):557-67 PMID: 5820642
  21. Properties of yeast pyruvate decarboxylase and their modification by proteolytic enzymes. II. Selective alteration by yeast proteases.
    Arch Biochem Biophys. 1968 Sep 20;127(1):89-100 PMID: 5681431
  22. X-ray and meiotic fine structure mapping of the adenine-8 locus in Saccharomyces cerevisiae.
    Genetics. 1968 Apr;58(4):507-27 PMID: 5685163
  23. The location of chitin in the yeast cell wall.
    Biochem J. 1966 Nov;101(2):36C-38C PMID: 5966273
  24. A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae.
    Genetics. 1966 Mar;53(3):445-59 PMID: 5919326
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.5.2395
Published
1979-05-00
Pages
2395-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383608
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.5.2395 PMC Full Text View journal details
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