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PMID: 2582352 Published · ppublish English Journal Article

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Nucleic acids research ·Vol. 13 ·No. 1 ·1985-01-11 ·Pages 45-57

Kempe T, Sundquist WI, Chow F, Hu SL

Abstract

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.

MeSH Terms
Biotin/analogs & derivatives,chemical synthesis Chemical Phenomena Chemistry Collodion DNA/metabolism DNA Ligases Nucleic Acid Hybridization Oligodeoxyribonucleotides/genetics,isolation & purification Oligonucleotides/genetics,isolation & purification RNA/metabolism RNA Ligase (ATP)
Chemicals
Oligodeoxyribonucleotides Oligonucleotides RNA Biotin Collodion DNA 2-(biotinylamido)ethanol DNA Ligases RNA Ligase (ATP)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kempe T
Sundquist W I
Chow F
Hu S L
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.1.45
Published
1985-01-11
Pages
45-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340973
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.1.45 PMC Full Text View journal details
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