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PMID: 2579101 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Human macrophage activation. Modulation of mannosyl, fucosyl receptor activity in vitro by lymphokines, gamma and alpha interferons, and dexamethasone.

The Journal of clinical investigation ·Vol. 75 ·No. 2 ·1985-02-00 ·Pages 624-31

Mokoena T, Gordon S

Abstract

We describe a sensitive assay to measure immune activation of human macrophages in cell culture. Freshly isolated blood monocytes from normal subjects lack the ability to endocytose and degrade mannosyl-terminated glycoconjugates via specific receptors, but acquired this activity after cultivation in autologous serum for approximately 3 d. Addition of specific antigen, purified protein derivative, or T cell mitogens to mononuclear cells prevented the appearance of macrophage mannosyl receptor activity and lymphokine, gamma-, and alpha-interferons selectively down-regulated receptor activity in monocyte-macrophage targets. The effects of antigen challenge and gamma-interferon on mannosyl receptors can be prevented by 10(-8) M dexamethasone. Dexamethasone also inhibited release of another macrophage activation marker, plasminogen activator, which was increased by both gamma- and alpha-interferons. These studies show that activation of human macrophages is regulated by opposing actions of lymphokines and glucocorticoids.

MeSH Terms
Adult Antigens/immunology Dexamethasone/pharmacology Fibrinolysis/drug effects Humans In Vitro Techniques Interferons/pharmacology Lectins, C-Type Lymphocyte Activation Lymphokines/pharmacology Macrophage Activation/drug effects Mannose Receptor Mannose-Binding Lectins Mitogens/pharmacology Monocytes/physiology Receptors, Cell Surface Receptors, Immunologic/drug effects,metabolism
Chemicals
Antigens Lectins, C-Type Lymphokines Mannose Receptor Mannose-Binding Lectins Mitogens Receptors, Cell Surface Receptors, Immunologic Dexamethasone Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mokoena T
Gordon S
References (37)
37 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111740
Published
1985-02-00
Pages
624-31
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423539
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI111740 PMC Full Text View journal details
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