Effect of phenethyl alcohol on Staphylococcus aureus alpha-lysin production.

Infection and immunity ·Vol. 47 ·No. 1 ·1985-01-00 ·Pages 112-7

Lee KY, Birkbeck TH

Abstract

Phenethyl alcohol, at the maximum concentration which did not inhibit growth (0.3% [vol/vol]), inhibited the production of alpha-lysin and exoproteases but not that of delta-lysin in Staphylococcus aureus Wood 46. The inhibition of alpha-lysin was reversible, and transient accumulation of cell-associated alpha-lysin occurred in the presence of PEA. A precursor of alpha-lysin ca. 3,000 daltons larger than extracellular alpha-lysin was immunologically detected in the sodium dodecyl sulfate extracts of membranes and whole cells of phenethyl alcohol-treated S. aureus cultures. Also, a degraded form of alpha-lysin was detected in membranes prepared from cells lysed by lysostaphin but not in membranes from cells lysed with an X-press.

MeSH Terms

Antigen-Antibody Complex Bacterial Toxins/biosynthesis,isolation & purification Cell Membrane/analysis Electrophoresis, Polyacrylamide Gel Ethanol/analogs & derivatives Hemolysin Proteins Immune Sera Kinetics Molecular Weight Phenylethyl Alcohol/pharmacology Staphylococcus aureus/drug effects,physiology

Chemicals

Antigen-Antibody Complex Bacterial Toxins Hemolysin Proteins Immune Sera staphylococcal alpha-toxin Ethanol Phenylethyl Alcohol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lee K Y

Birkbeck T H

References (29)

29 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.47.1.112-117.1985

Published

1985-01-00

Pages

112-7

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC261484

Subset

External Links

PubMed source DOI: 10.1128/iai.47.1.112-117.1985 PMC Full Text View journal details

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Effect of phenethyl alcohol on Staphylococcus aureus alpha-lysin production.

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