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PMID: 2565041 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of ATP-sensitive K+ channels in insulinoma cells: activation by somatostatin and protein kinase C and the role of cAMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 8 ·1989-04-00 ·Pages 2971-5

de Weille JR, Schmid-Antomarchi H, Fosset M, Lazdunski M

Abstract

The actions of somatostatin and of the phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) were studied in rat insulinoma (RINm5F) cells by electrophysiological and 86Rb+ flux techniques. Both PMA and somatostatin hyperpolarize insulinoma cells by activating ATP-sensitive K+ channels. The presence of intracellular GTP is required for the somatostatin effects. PMA- and somatostatin-induced hyperpolarization and channel activity are inhibited by the sulfonylurea glibenclamide. Glibenclamide-sensitive 86Rb+ efflux from insulinoma cells is stimulated by somatostatin in a dose-dependent manner (half maximal effect at 0.7 nM) and abolished by pertussis toxin pretreatment. Mutual roles of a GTP-binding protein, of protein kinase C, and of cAMP in the regulation of ATP-sensitive K+ channels are discussed.

MeSH Terms
Adenoma, Islet Cell/physiopathology Adenosine Triphosphate/physiology Animals Cyclic AMP/physiology Glyburide/pharmacology Insulinoma/physiopathology Pertussis Toxin Potassium Channels/physiology Protein Kinase C/physiology Rats Rubidium/metabolism Somatostatin/pharmacology Tetradecanoylphorbol Acetate/pharmacology Tumor Cells, Cultured Virulence Factors, Bordetella/pharmacology
Chemicals
Potassium Channels Virulence Factors, Bordetella Somatostatin Adenosine Triphosphate Cyclic AMP Pertussis Toxin Protein Kinase C Rubidium Tetradecanoylphorbol Acetate Glyburide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Weille J R
Centre de Biochimie du Centre National de la Recherche Scientifique, Nice, France.
Schmid-Antomarchi H
Fosset M
Lazdunski M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.8.2971
Published
1989-04-00
Pages
2971-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287042
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.8.2971 PMC Full Text View journal details
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