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PMID: 25618 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of cell wall polymers secreted into the growth medium of lysis-defective pneumococci during treatment with penicillin and other inhibitors of cell wall synthesis.

Antimicrobial agents and chemotherapy ·Vol. 13 ·No. 2 ·1978-02-00 ·Pages 302-11

Hakenbeck R, Waks S, Tomasz A

Abstract

Autolysin-defective pneumococci secrete large quantities of choline-containing cell wall polymers into the growth medium during treatment with inhibitors of peptidoglycan synthesis. The secreted polymers were separated into three fractions by a combination of gel filtration on agarose and sodium dodecyl sulfate-gel electrophoresis. Fraction I had a high apparent molecular size and contained the Forssman antigen in complex with material exhibiting properties of cell wall teichoic acid. Choline-containing polymers of as yet uncharacterized structure were present in both fractions IIA and IIB, and fraction IIA also contained peptidoglycan components.

MeSH Terms
Antibodies, Bacterial Cell Wall/drug effects,metabolism Cycloserine/pharmacology Penicillins/pharmacology Polymers/analysis Polysaccharides, Bacterial/immunology Streptococcus pneumoniae/drug effects,metabolism,ultrastructure
Chemicals
Antibodies, Bacterial Penicillins Polymers Polysaccharides, Bacterial Cycloserine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hakenbeck R
Waks S
Tomasz A
References (27)
27 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.13.2.302
Published
1978-02-00
Pages
302-11
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC352231
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.13.2.302 PMC Full Text View journal details
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