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PMID: 2557846 Published · ppublish English Journal Article

Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil.

The Biochemical journal ·Vol. 264 ·No. 2 ·1989-12-01 ·Pages 617-20

Bonser RW, Thompson NT, Randall RW, Garland LG

Abstract

Neutrophils stimulated with formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) in the presence of butanol and ethanol formed phosphatidyl alcohols through a phospholipase D mechanism. The alcohols inhibited phosphatidic acid and diradylglycerol (DRG) formation, but did not block inositol 1, 4, 5-trisphosphate release. fMet-Leu-Phe-stimulated superoxide production was inhibited by alcohol concentrations which blocked DRG formation, whereas opsonized-zymosan-stimulated superoxide production was only partially decreased. These results suggest that phospholipase D activation is functionally linked to superoxide production in the human neutrophil.

MeSH Terms
1-Butanol Butanols/pharmacology Enzyme Activation Ethanol/pharmacology Humans In Vitro Techniques Kinetics N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/drug effects,enzymology,physiology Phospholipase D/blood Phospholipases/blood Phospholipids/blood Superoxides/blood
Chemicals
Butanols Phospholipids Superoxides Ethanol N-Formylmethionine Leucyl-Phenylalanine 1-Butanol Phospholipases Phospholipase D
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bonser R W
Department of Biochemical Sciences, Wellcome Research Laboratories, Beckenham, Kent, U.K.
Thompson N T
Randall R W
Garland L G
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2640617
Published
1989-12-01
Pages
617-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133625
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1042/bj2640617 PMC Full Text View journal details
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