Home LiteratureArticle Details
PMID: 2555162 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phospholipase C-gamma, a substrate for PDGF receptor kinase, is not phosphorylated on tyrosine during the mitogenic response to CSF-1.

The EMBO journal ·Vol. 8 ·No. 11 ·1989-11-00 ·Pages 3345-50

Downing JR, Margolis BL, Zilberstein A, Ashmun RA, Ullrich A, Sherr CJ, Schlessinger J

Abstract

Quiescent mouse NIH3T3 cells expressing a transduced human c-fms gene encoding the receptor for colony stimulating factor-1 (CSF-1) were stimulated with mitogenic concentrations of platelet-derived growth factor (PDGF) or CSF-1. Immunoprecipitated phospholipase C-gamma (PLC-gamma) was phosphorylated on tyrosine and calcium was mobilized following treatment of intact cells with PDGF. In contrast, only trace amounts of phosphotyrosine were incorporated into PLC-gamma and no intracellular calcium signal was detected after CSF-1 stimulation. Similarly, CSF-1 treatment did not stimulate phosphorylation of PLC-gamma on tyrosine in a CSF-1-dependent. SV40-immortalized mouse macrophage cell line that expresses high levels of the CSF-1 receptor. In fibroblasts, antiserum to PLC-gamma co-precipitated a fraction of the tyrosine phosphorylated form of the PDGF receptor (PDGF-R) after ligand stimulation, implying that phosphorylated PDGF-R and PLC-gamma were associated in a stable complex. Pre-treatment of cells with orthovanadate also led to tyrosine phosphorylation of PLC-gamma which was significantly enhanced by PDGF, but not by CSF-1. Thus, although the PDGF and CSF-1 receptors are structurally related and appear to be derived from a single ancestor gene, only PDGF-induced mitogenesis in fibroblasts correlated with tyrosine phosphorylation of PLC-gamma.

MeSH Terms
Animals Calcium/metabolism Cell Line Colony-Stimulating Factors/pharmacology Humans Macrophage Colony-Stimulating Factor Macrophages/metabolism Mice Mitogens/pharmacology Phosphorylation Platelet-Derived Growth Factor/pharmacology Precipitin Tests Protein-Tyrosine Kinases/metabolism,pharmacology Proto-Oncogenes Receptors, Cell Surface/metabolism Receptors, Colony-Stimulating Factor Receptors, Platelet-Derived Growth Factor Type C Phospholipases/metabolism Tyrosine/metabolism
Chemicals
Colony-Stimulating Factors Mitogens Platelet-Derived Growth Factor Receptors, Cell Surface Receptors, Colony-Stimulating Factor Tyrosine Macrophage Colony-Stimulating Factor Protein-Tyrosine Kinases Receptors, Platelet-Derived Growth Factor Type C Phospholipases Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Downing J R
Department of Tumor Cell Biology, St Jude Children's Research Hospital, Memphis, TN 38105.
Margolis B L
Zilberstein A
Ashmun R A
Ullrich A
Sherr C J
Schlessinger J
References (45)
45 references, click to expand
  1. Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
    Nature. 1982 Aug 12;298(5875):667-9 PMID: 6178977
  2. Platelet-derived growth factor. II. Specific binding to cultured cells.
    J Biol Chem. 1982 May 10;257(9):5161-71 PMID: 6279659
  3. Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.
    J Biol Chem. 1983 Aug 25;258(16):9581-4 PMID: 6309774
  4. CSF-1--a mononuclear phagocyte lineage-specific hemopoietic growth factor.
    J Cell Biochem. 1983;21(2):151-9 PMID: 6309875
  5. Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):85-9 PMID: 6582485
  6. Subcellular localization of glycoproteins encoded by the viral oncogene v-fms.
    J Virol. 1984 Sep;51(3):730-41 PMID: 6381756
  7. Cell surface expression of v-fms-coded glycoproteins is required for transformation.
    Mol Cell Biol. 1984 Oct;4(10):1999-2009 PMID: 6390182
  8. The product of the c-fms proto-oncogene: a glycoprotein with associated tyrosine kinase activity.
    Science. 1985 Apr 19;228(4697):320-2 PMID: 2580348
  9. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1.
    Cell. 1985 Jul;41(3):665-76 PMID: 2408759
  10. A guanine nucleotide-dependent phosphatidylinositol 4,5-diphosphate phospholipase C in cells transformed by the v-fms and v-fes oncogenes.
    J Biol Chem. 1986 Apr 15;261(11):4978-85 PMID: 3007485
  11. Structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus.
    Nature. 1986 Mar 20-26;320(6059):277-80 PMID: 2421165
  12. A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family.
    Nature. 1986 Apr 3-9;320(6061):415-21 PMID: 3007997
  13. The biology of platelet-derived growth factor.
    Cell. 1986 Jul 18;46(2):155-69 PMID: 3013421
  14. Antibodies to distal carboxyl terminal epitopes in the v-fms-coded glycoprotein do not cross-react with the c-fms gene product.
    Virology. 1986 Jul 30;152(2):432-45 PMID: 2425481
  15. Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors.
    Nature. 1986 Sep 18-24;323(6085):226-32 PMID: 3020426
  16. Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media.
    Mol Cell Biol. 1986 Oct;6(10):3541-4 PMID: 3796592
  17. Epidermal growth factor stimulates the production of phosphatidylinositol monophosphate and the breakdown of polyphosphoinositides in A431 cells.
    J Biol Chem. 1987 Feb 5;262(4):1644-51 PMID: 2433285
  18. Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
    Nature. 1987 Feb 5-11;325(6104):549-52 PMID: 3027579
  19. The haemopoietic growth factors interleukin 3 and colony stimulating factor-1 stimulate proliferation but do not induce inositol lipid breakdown in murine bone-marrow-derived macrophages.
    EMBO J. 1986 Dec 1;5(12):3281-6 PMID: 3493133
  20. Epidermal growth factor stimulates the rapid accumulation of inositol (1,4,5)-trisphosphate and a rise in cytosolic calcium mobilized from intracellular stores in A431 cells.
    J Biol Chem. 1987 Mar 5;262(7):2951-6 PMID: 3102480
  21. Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5.
    J Cell Physiol. 1987 Mar;130(3):420-7 PMID: 3031090
  22. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  23. Bovine brain cytosol contains three immunologically distinct forms of inositolphospholipid-specific phospholipase C.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6649-53 PMID: 3477795
  24. Epidermal growth factor and angiotensin II stimulate formation of inositol 1,4,5- and inositol 1,3,4-trisphosphate in hepatocytes. Differential inhibition by pertussis toxin and phorbol 12-myristate 13-acetate.
    J Biol Chem. 1987 Dec 25;262(36):17285-93 PMID: 3500949
  25. Epidermal growth factor stimulates formation of inositol phosphates in BALB/c/3T3 cells pretreated with cholera toxin and isobutylmethylxanthine.
    J Biol Chem. 1988 Jan 25;263(3):1111-4 PMID: 2447085
  26. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.
    EMBO J. 1987 Nov;6(11):3341-51 PMID: 2448137
  27. Ligand-induced tyrosine kinase activity of the colony-stimulating factor 1 receptor in a murine macrophage cell line.
    Mol Cell Biol. 1988 Apr;8(4):1795-9 PMID: 2837654
  28. A point mutation at the ATP-binding site of the EGF-receptor abolishes signal transduction.
    EMBO J. 1988 Mar;7(3):707-10 PMID: 3260862
  29. Antiphosphotyrosine recovery of phospholipase C activity after EGF treatment of A-431 cells.
    Science. 1988 Aug 19;241(4868):968-70 PMID: 2457254
  30. Ligand activation causes a phosphorylation-dependent change in platelet-derived growth factor receptor conformation.
    J Biol Chem. 1988 Sep 15;263(26):12805-8 PMID: 2843499
  31. Monoclonal antibodies to three phospholipase C isozymes from bovine brain.
    J Biol Chem. 1988 Oct 5;263(28):14497-504 PMID: 2459119
  32. Tandem linkage of human CSF-1 receptor (c-fms) and PDGF receptor genes.
    Cell. 1988 Nov 18;55(4):655-61 PMID: 2846185
  33. A point mutation in the extracellular domain of the human CSF-1 receptor (c-fms proto-oncogene product) activates its transforming potential.
    Cell. 1988 Dec 23;55(6):979-88 PMID: 2974321
  34. Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes.
    Science. 1989 Feb 10;243(4892):800-4 PMID: 2536956
  35. Epidermal growth factor stimulates tyrosine phosphorylation of phospholipase C-II independently of receptor internalization and extracellular calcium.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1568-72 PMID: 2466293
  36. Signal transduction by the platelet-derived growth factor receptor.
    Science. 1989 Mar 24;243(4898):1564-70 PMID: 2538922
  37. Studies of inositol phospholipid-specific phospholipase C.
    Science. 1989 May 5;244(4904):546-50 PMID: 2541501
  38. EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling.
    Cell. 1989 Jun 30;57(7):1101-7 PMID: 2472218
  39. Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
    Cell. 1989 Jun 30;57(7):1109-22 PMID: 2472219
  40. Platelet-derived growth factor induces rapid and sustained tyrosine phosphorylation of phospholipase C-gamma in quiescent BALB/c 3T3 cells.
    Mol Cell Biol. 1989 Jul;9(7):2934-43 PMID: 2550789
  41. Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
    J Biol Chem. 1980 Sep 25;255(18):8363-5 PMID: 6157683
  42. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  43. Early changes in phosphatidylinositol and arachidonic acid metabolism in quiescent swiss 3T3 cells stimulated to divide by platelet-derived growth factor.
    J Biol Chem. 1981 Dec 10;256(23):12329-35 PMID: 6795201
  44. Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
    Nature. 1982 Feb 4;295(5848):419-20 PMID: 6173766
  45. Platelet-derived growth factor stimulates tyrosine-specific protein kinase activity in Swiss mouse 3T3 cell membranes.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4303-7 PMID: 6181505
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-11-00
Pages
3345-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401473
Subset
IM
Grants
NCI NIH HHS · CA-01429 · United States
NCI NIH HHS · CA-21765 · United States
NCI NIH HHS · CA-47064 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2