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PMID: 25550508 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 112 ·No. 2 ·2015-01-13 ·Pages 613-8

Wang P, Du Y, Hou YJ, Zhao Y, Hsu CC, Yuan F, Zhu X, Tao WA, Song CP, Zhu JK

Abstract

The phytohormone abscisic acid (ABA) plays important roles in plant development and adaptation to environmental stress. ABA induces the production of nitric oxide (NO) in guard cells, but how NO regulates ABA signaling is not understood. Here, we show that NO negatively regulates ABA signaling in guard cells by inhibiting open stomata 1 (OST1)/sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6) through S-nitrosylation. We found that SnRK2.6 is S-nitrosylated at cysteine 137, a residue adjacent to the kinase catalytic site. Dysfunction in the S-nitrosoglutathione (GSNO) reductase (GSNOR) gene in the gsnor1-3 mutant causes NO overaccumulation in guard cells, constitutive S-nitrosylation of SnRK2.6, and impairment of ABA-induced stomatal closure. Introduction of the Cys137 to Ser mutated SnRK2.6 into the gsnor1-3/ost1-3 double-mutant partially suppressed the effect of gsnor1-3 on ABA-induced stomatal closure. A cysteine residue corresponding to Cys137 of SnRK2.6 is present in several yeast and human protein kinases and can be S-nitrosylated, suggesting that the S-nitrosylation may be an evolutionarily conserved mechanism for protein kinase regulation.

Keywords
ABA GSNOR NO drought stomata
MeSH Terms
Abscisic Acid/metabolism Amino Acid Sequence Amino Acid Substitution Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Catalytic Domain/genetics Conserved Sequence Cysteine/chemistry Glutathione Reductase/genetics,metabolism Humans Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Nitric Oxide/metabolism Nitric Oxide Donors/pharmacology Phenotype Plant Stomata/cytology,metabolism Protein Conformation Protein Kinase Inhibitors/pharmacology Protein Kinases/chemistry,genetics,metabolism S-Nitrosoglutathione/pharmacology Sequence Homology, Amino Acid Signal Transduction
Chemicals
Arabidopsis Proteins Nitric Oxide Donors Protein Kinase Inhibitors Nitric Oxide S-Nitrosoglutathione Abscisic Acid Glutathione Reductase S-nitrosoglutathione reductase, Arabidopsis Protein Kinases OST1 protein, Arabidopsis Cysteine
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wang Pengcheng
Department of Horticulture and Landscape Architecture and.
Du Yanyan
Department of Horticulture and Landscape Architecture and Department of Biology, Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475001, China; and.
Hou Yueh-Ju
Department of Horticulture and Landscape Architecture and.
Zhao Yang
Department of Horticulture and Landscape Architecture and Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Hsu Chuan-Chih
Department of Biochemistry, Purdue University, West Lafayette, IN 47907;
Yuan Feijuan
Department of Biology, Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475001, China; and.
Zhu Xiaohong
Department of Horticulture and Landscape Architecture and.
Tao W Andy
Department of Biochemistry, Purdue University, West Lafayette, IN 47907;
Song Chun-Peng
Department of Biology, Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475001, China; and.
Zhu Jian-Kang
Department of Horticulture and Landscape Architecture and Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China jkzhu@purdue.edu.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1423481112
Email
jkzhu@purdue.edu
Published
2015-01-13
Epub
2014-00-30
Pages
613-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC4299189
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01GM059138 · United States
Corrections
CommentIn
PMID: 25682398
External Links
PubMed source DOI: 10.1073/pnas.1423481112 PMC Full Text View journal details
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