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PMID: 2548089 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Splicing of a yeast intron containing an unusual 5' junction sequence.

Molecular and cellular biology ·Vol. 9 ·No. 6 ·1989-06-00 ·Pages 2765-70

Hodge MR, Cumsky MG

Abstract

The Saccharomyces cerevisiae COX5b gene contains a small intron that is unique in two respects. First, it interrupts the ATG codon that initiates translation of the COX5b product. Second, it contains a sequence at the 5' splice junction (5'-GCATGT-3') that differs from the highly conserved yeast hexanucleotide (5'-GTAPyGT-3') and from the 5'-GT found at the corresponding position in nearly all introns of eucaryotic protein-coding genes. We have analyzed both the transcripts derived from the COX5b gene and the splicing of its intron. We show here that an unspliced mRNA precursor constituted a minor fraction of the total COX5b message, even when the gene was overexpressed. We also show that both major transcripts derived from COX5b had been spliced. Our results suggest that at least in the case of COX5b, a 5'-GC can function as efficiently as the highly conserved 5'-GT in the splicing reaction.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics Electron Transport Complex IV/genetics Introns Molecular Sequence Data Mutation Plasmids RNA Precursors/genetics,metabolism RNA Splicing RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
RNA Precursors RNA, Messenger DNA Electron Transport Complex IV
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hodge M R
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Cumsky M G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.6.2765-2770.1989
Published
1989-06-00
Pages
2765-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362354
Subset
IM
Grants
NIGMS NIH HHS · GM071443-14 · United States
NIGMS NIH HHS · GM36675 · United States
Databases
GENBANK
M27074
External Links
PubMed source DOI: 10.1128/mcb.9.6.2765-2770.1989 PMC Full Text View journal details
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