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PMID: 2541616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Establishment of a human in vitro mesothelial cell model system for investigating mechanisms of asbestos-induced mesothelioma.

The American journal of pathology ·Vol. 134 ·No. 5 ·1989-05-00 ·Pages 979-91

Ke Y, Reddel RR, Gerwin BI, Reddel HK, Somers AN, McMenamin MG, LaVeck MA, Stahel RA, Lechner JF, Harris CC

Abstract

Normal human mesothelial (NHM) cells were transfected with a plasmid containing SV40 early region DNA. Individual colonies of transformed cells from several donors were subcultured for periods of 5 to 6 months and 60 to 70 population doublings (PDs) before senescence, in contrast to a culture lifespan of approximately 1 month and 15 PDs for NHM cells. One such culture, designated MeT-5A, escaped senescence and has been passaged continuously for more than 2 years. These cells had a single integrated copy of SV40 early region DNA in their genome, expressed SV40 large T antigen, and exhibited features of mesothelial cells including sensitivity to the cytotoxic effects of asbestos fibers. One year after injection subcutaneously or intraperitoneally in athymic nude mice, these cells remain nontumorigenic, and therefore are a potential model system for in vitro fiber carcinogenesis studies.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/analysis Asbestos/adverse effects,pharmacology Asbestos, Amosite Carcinogenicity Tests Cell Survival Cell Transformation, Neoplastic Cell Transformation, Viral Cells, Cultured Glycoproteins/genetics Growth Substances/pharmacology Humans Karyotyping Mesothelioma/etiology,genetics,pathology Mice Mice, Nude Plasmids Plasminogen Activators/antagonists & inhibitors Plasminogen Inactivators Pleural Neoplasms/etiology,genetics,pathology RNA, Messenger/analysis Simian virus 40/immunology,physiology Transfection
Chemicals
Antigens, Polyomavirus Transforming Glycoproteins Growth Substances Plasminogen Inactivators RNA, Messenger Asbestos, Amosite Asbestos Plasminogen Activators
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ke Y
Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892.
Reddel R R
Gerwin B I
Reddel H K
Somers A N
McMenamin M G
LaVeck M A
Stahel R A
Lechner J F
Harris C C
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1989-05-00
Pages
979-91
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1879894
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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