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PMID: 2540158 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control mechanism of the Escherichia coli K-12 cell cycle is triggered by the cyclic AMP-cyclic AMP receptor protein complex.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2909-12

Utsumi R, Noda M, Kawamukai M, Komano T

Abstract

The role of cyclic AMP (cAMP) in the cell cycle of Escherichia coli K-12 was studied in three mutant strains. One was KI1812, in which the cya promoter is replaced by the lacUV5 promoter. In KI1812, isopropyl-beta-D-thiogalactopyranoside induced the synthesis of cya mRNA, and at the same time cell division was inhibited and short filaments containing multiple nuclei were formed. The other strains were constructed as double mutants (NC6707 cya sulB [ftsZ(Ts)] and TR3318 crp sulB [ftsZ(Ts)]). In both double mutants, filamentation was repressed at 42 degrees C, but it was induced again by addition of cAMP in strain NC6707 and introduction of pHA7 containing wild-type crp in TR3318. These results indicate that lateral wall synthesis in the E. coli cell cycle is triggered by the cAMP-cAMP receptor protein complex.

MeSH Terms
Cell Cycle Cell Wall/ultrastructure Cyclic AMP/physiology Escherichia coli/cytology,physiology Gene Expression Regulation Mutation Peptidoglycan/biosynthesis Receptors, Cyclic AMP/physiology
Chemicals
Peptidoglycan Receptors, Cyclic AMP Cyclic AMP
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Utsumi R
Department of Agricultural Chemistry, Kinki University, Higashiosaka, Japan.
Noda M
Kawamukai M
Komano T
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2909-2912.1989
Published
1989-05-00
Pages
2909-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209987
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.5.2909-2912.1989 PMC Full Text View journal details
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