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PMID: 2540151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic analysis of the rnc operon of Escherichia coli.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2581-90

Takiff HE, Chen SM, Court DL

Abstract

RNase III, an Escherichia coli double-stranded endoribonuclease, is known to be involved in maturation of rRNA and regulation of several bacteriophage and Escherichia coli genes. Clones of the region of the E. coli chromosome containing the gene for RNase III (rnc) were obtained by screening genomic libraries in lambda with DNA known to map near rnc. A phage clone with the rnc region was randomly mutagenized with a delta Tn10 element, and the insertions were recombined onto the chromosome, generating a series of strains with delta Tn10 insertions in the rnc region. Two insertions that had Rnc- phenotypes were located. One of them lay in the rnc gene, and one was in the rnc leader sequence. Polarity studies showed that rnc is in an operon with two other genes, era and recO. The sequence of the recO gene beyond era indicated it could encode a protein of approximately 26 kilodaltons and, like rnc and era, had codon usage consistent with a low level of expression. Experiments using antibiotic cassettes to disrupt the genes rnc, era, and recO showed that era is essential for E. coli growth but that rnc and recO are dispensable.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA Mutational Analysis DNA Transposable Elements Endoribonucleases/physiology Escherichia coli/genetics Escherichia coli Proteins GTP-Binding Proteins/genetics Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Operon Restriction Mapping Ribonuclease III
Chemicals
Bacterial Proteins DNA Transposable Elements Escherichia coli Proteins Endoribonucleases Ribonuclease III ribonuclease III, E coli GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takiff H E
Laboratory of Molecular Oncology, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701.
Chen S M
Court D L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2581-2590.1989
Published
1989-05-00
Pages
2581-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209937
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
M26415, M26416
External Links
PubMed source DOI: 10.1128/jb.171.5.2581-2590.1989 PMC Full Text View journal details
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