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PMID: 2538831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transactivation of both early and late simian virus 40 promoters by large tumor antigen does not require nuclear localization of the protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 7 ·1989-04-00 ·Pages 2123-7

Wildeman AG

Abstract

The early gene product of simian virus 40, large tumor antigen (T antigen), is required for the onset of viral replication. This protein has also been reported to transactivate viral late gene expression, independently of replication. In this study I have used a vector that permits simultaneously a precise quantitation of simian virus 40 early and late promoter activity with a single nuclease S1 mapping probe. The results show that T antigen can activate the early promoter as well as the late promoter and that only on replicating templates does a shift occur in the ratio of late-to-early transcription. This simultaneous transactivation of early and late promoters occurs in human (HeLa) and monkey (CV-1) cells but does not occur in mouse embryonal carcinoma cells. It is seen with either wild-type T antigen or with a T antigen protein that carries a mutation in the nuclear localization signal. The mutant protein cannot bring about an early-to-late shift, consistent with its inability to support viral replication.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics Cell Line Cell Nucleus/metabolism Gene Expression Regulation Genes, Viral Genetic Vectors HeLa Cells/metabolism Humans Plasmids Promoter Regions, Genetic Restriction Mapping Simian virus 40/genetics,physiology Transcription, Genetic Virus Replication
Chemicals
Antigens, Polyomavirus Transforming
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wildeman A G
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.7.2123
Published
1989-04-00
Pages
2123-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286863
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.7.2123 PMC Full Text View journal details
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