Home LiteratureArticle Details
PMID: 2536837 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of the major capsid protein gene of human cytomegalovirus.

Journal of virology ·Vol. 63 ·No. 3 ·1989-03-00 ·Pages 1345-53

Chee M, Rudolph SA, Plachter B, Barrell B, Jahn G

Abstract

The coding region for the major capsid protein (MCP) of human cytomegalovirus (HCMV) was identified by comparing the protein sequence with the respective sequences of herpes simplex virus (HSV), Epstein-Barr virus, and varicella-zoster virus. The predicted length of the HCMV MCP was 1,370 amino acids. Comparison of the MCP sequences of the different human herpesviruses showed a homology of 25% to the MCP of HSV type 1, a homology of 29% to the MCP of Epstein-Barr virus, and a homology of 23% to the MCP of varicella-zoster virus. A subfragment of the HSV type 1 KpnI i fragment encoding the MCP VP5 cross-hybridized with the HCMV HindIII U fragment containing part of the MCP gene. Northern (RNA) blot analyses with subclones out of the coding region for the HCMV MCP detected one large transcript of about 8 kilobases. A portion of the open reading frame was expressed in Escherichia coli plasmid pBD2 IC2OH as a beta-galactosidase fusion protein and was used to generate polyclonal antibodies in New Zealand White rabbits. The obtained antisera reacted in Western immunoblots with the MCP of purified HCMV virions. A monoclonal antibody against the human MCP and a monospecific rabbit antiserum against strain Colburn of simian cytomegalovirus detected the fusion protein as well as the MCP of purified virions in immunoblots.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Western Capsid/genetics Cloning, Molecular Cytomegalovirus/genetics,immunology Genes, Viral Molecular Sequence Data RNA, Messenger/genetics Recombinant Fusion Proteins/immunology Restriction Mapping
Chemicals
RNA, Messenger Recombinant Fusion Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chee M
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Rudolph S A
Plachter B
Barrell B
Jahn G
References (53)
53 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.
    J Virol. 1978 May;26(2):389-410 PMID: 207894
  3. Analysis of structural polypeptides of purified human cytomegalovirus.
    J Virol. 1976 Dec;20(3):604-11 PMID: 186638
  4. Human cytomegalovirus: purification of enveloped virions and dense bodies.
    J Gen Virol. 1977 Aug;36(2):345-9 PMID: 197204
  5. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
  6. Physical mapping of herpes simplex virus-induced polypeptides.
    J Virol. 1978 Nov;28(2):624-42 PMID: 214583
  7. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  8. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  9. A new computer method for the storage and manipulation of DNA gel reading data.
    Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
  10. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
    Science. 1981 Mar 27;211(4489):1437-8 PMID: 6162199
  11. Identification of a primate cytomegalovirus group-common protein antigen.
    Virology. 1981 Nov;115(1):182-91 PMID: 6270897
  12. Use of recombinant plasmids to investigate the structure of the human cytomegalovirus genome.
    J Gen Virol. 1982 Mar;59(Pt 1):111-29 PMID: 6279770
  13. Cloning of the complete human cytomegalovirus genome in cosmids.
    Gene. 1982 Apr;18(1):39-46 PMID: 6286417
  14. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  15. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  16. Protein counterparts of human and simian cytomegaloviruses.
    Virology. 1983 Jul 30;128(2):391-406 PMID: 6310864
  17. Relative titers of antibodies to individual polypeptide antigens of herpes simplex virus type 1 in human sera.
    J Infect Dis. 1983 Sep;148(3):436-44 PMID: 6311910
  18. Isolation and characterization of a noninfectious virion-like particle released from cells infected with human strains of cytomegalovirus.
    Virology. 1983 Oct 15;130(1):118-33 PMID: 6314643
  19. Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies.
    Anal Biochem. 1983 Aug;133(1):214-9 PMID: 6356979
  20. Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5.
    J Virol. 1984 Jan;49(1):287-92 PMID: 6317894
  21. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  22. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  23. Characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins.
    Virology. 1984 Jan 30;132(2):325-38 PMID: 6322416
  24. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  25. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  26. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.
    Gene. 1984 Dec;32(3):481-5 PMID: 6099327
  27. The current status and portability of our sequence handling software.
    Nucleic Acids Res. 1986 Jan 10;14(1):217-31 PMID: 3511446
  28. Regulation of cytomegalovirus late gene expression: gamma genes are controlled by posttranscriptional events.
    J Virol. 1986 Mar;57(3):864-74 PMID: 3005633
  29. Mapping of the major glycoprotein gene of human cytomegalovirus.
    J Gen Virol. 1986 Jul;67 ( Pt 7):1461-7 PMID: 2873201
  30. Characterization of phosphoproteins and protein kinase activity of virions, noninfectious enveloped particles, and dense bodies of human cytomegalovirus.
    J Virol. 1986 Sep;59(3):714-27 PMID: 3016333
  31. A cis-acting element within the 5' leader of a cytomegalovirus beta transcript determines kinetic class.
    Cell. 1986 Sep 12;46(6):865-72 PMID: 3019554
  32. DNA sequence of the major capsid protein gene of herpes simplex virus type 1.
    J Gen Virol. 1986 Oct;67 ( Pt 10):2279-86 PMID: 3020164
  33. Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene.
    J Virol. 1987 Jan;61(1):119-24 PMID: 3023689
  34. Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene.
    J Virol. 1987 Jan;61(1):125-33 PMID: 3023690
  35. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.
    EMBO J. 1986 Nov;5(11):3057-63 PMID: 3024973
  36. Expression of a human cytomegalovirus late gene is posttranscriptionally regulated by a 3'-end-processing event occurring exclusively late after infection.
    Mol Cell Biol. 1986 Dec;6(12):4202-13 PMID: 3025644
  37. Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus.
    J Virol. 1987 Feb;61(2):446-53 PMID: 3027374
  38. Genetic relations between varicella-zoster virus and Epstein-Barr virus.
    J Gen Virol. 1987 Apr;68 ( Pt 4):1067-79 PMID: 3033126
  39. The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties.
    J Gen Virol. 1987 May;68 ( Pt 5):1327-37 PMID: 3033138
  40. Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus.
    J Virol. 1987 May;61(5):1358-67 PMID: 3033266
  41. A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
    J Mol Biol. 1987 Nov 20;198(2):327-37 PMID: 3430611
  42. Random cloning and sequencing by the M13/dideoxynucleotide chain termination method.
    Methods Enzymol. 1987;155:51-93 PMID: 3323821
  43. Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H.
    J Virol. 1988 Apr;62(4):1416-22 PMID: 2831402
  44. A multigene family encodes the human cytomegalovirus glycoprotein complex gcII (gp47-52 complex).
    J Virol. 1988 Jun;62(6):1956-62 PMID: 2835500
  45. Identification and procaryotic expression of the gene coding for the highly immunogenic 28-kilodalton structural phosphoprotein (pp28) of human cytomegalovirus.
    J Virol. 1988 Jul;62(7):2243-50 PMID: 2836608
  46. Prokaryotic expression of immunogenic polypeptides of the large phosphoprotein (pp150) of human cytomegalovirus.
    J Gen Virol. 1988 Jun;69 ( Pt 6):1195-204 PMID: 2455019
  47. Characterization of a human cytomegalovirus glycoprotein complex (gcI).
    J Gen Virol. 1988 Jun;69 ( Pt 6):1205-15 PMID: 2838571
  48. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.
    J Gen Virol. 1988 Jul;69 ( Pt 7):1531-74 PMID: 2839594
  49. Antigenic cross-reactions among herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus.
    J Virol. 1987 Apr;61(4):1125-35 PMID: 3029407
  50. Large-scale rearrangement of homologous regions in the genomes of HCMV and EBV.
    Virology. 1987 Apr;157(2):397-413 PMID: 3029980
  51. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  52. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  53. Human cytomegalovirus: glycoproteins associated with virions and dense bodies.
    J Virol. 1976 Aug;19(2):594-609 PMID: 183016
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.3.1345-1353.1989
Published
1989-03-00
Pages
1345-53
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247832
Subset
IM
Databases
GENBANK
M25411
External Links
PubMed source DOI: 10.1128/JVI.63.3.1345-1353.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2