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PMID: 2536354 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Centromeric association and non-random distribution of centromeres in human tumour cells.

Human genetics ·Vol. 81 ·No. 2 ·1989-01-00 ·Pages 137-43

Haaf T, Schmid M

Abstract

Centromere arrangement in interphase and metaphase cells of two human tumour cell lines was analysed using anti-kinetochore antibodies as immunofluorescent probes. In GLC1 interphase nuclei, kinetochores were non-randomly positioned around the nucleolus and close to the nuclear membrane. During S and early G2 phase, necklace-like strands of kinetochores were formed in the centre of the nucleus. The duplication of sister kinetochores during the G2 phase was not synchronized. At late G2 phase, a relatively random topological distribution of centromeres was observed with short linear arrays of sister kinetochores. Carefully spread metaphase plates of MDA-MB231 cells generally exhibited a linear alignment of centromeres and large centromeric clusters. In completely pulverized MDA-MB231 cells, centromeres showed a strong tendency to associate with each other.

MeSH Terms

Breast Neoplasms/genetics Carcinoma, Small Cell/genetics Centromere Chromosome Aberrations Chromosomes Fluorescent Antibody Technique Humans Karyotyping Lung Neoplasms/genetics Metaphase Tumor Cells, Cultured/ultrastructure

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Haaf T

Institut für Humangenetik der Universität Würzburg, Federal Republic of Germany.

Schmid M

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27 references, click to expand

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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00293889

Published

1989-01-00

Pages

137-43

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00293889 View journal details

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Centromeric association and non-random distribution of centromeres in human tumour cells.

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