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PMID: 2536159 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 1 ·1989-01-00 ·Pages 51-5

Weil MD, McClelland M

Abstract

The circular genome of Staphylococcus aureus was cut into two fragments by a simple enzymatic method that cleaves a 10-base-pair site. The recognition sequence, A-T-C-G-mA decreases T-C-G-mA-T, was created by the combined use of the methylase M.Cla I (A-T-C-G-mA-T) and the restriction endonuclease Dpn I (G-mA decreases T-C). This technique is insensitive to CpG methylation and in human DNA is predicted to produce fragments that, on average, are greater than five million base pairs. The ability to create such long pieces of DNA should facilitate mapping of large, complex chromosomes.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes/metabolism Genes, Bacterial Molecular Sequence Data Staphylococcus aureus/genetics Substrate Specificity
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weil M D
Department of Biochemistry, University of Chicago, IL 60637.
McClelland M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.1.51
Published
1989-01-00
Pages
51-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286401
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.1.51 PMC Full Text View journal details
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