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PMID: 2531281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.

Molecular and cellular biology ·Vol. 9 ·No. 10 ·1989-10-00 ·Pages 4550-5

Tsai AY, Streuli M, Saito H

Abstract

By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.

MeSH Terms
Antigens, Differentiation/genetics B-Lymphocytes/analysis Base Sequence DNA Mutational Analysis Exons/physiology Genetic Vectors Histocompatibility Antigens/genetics Humans Introns/physiology Leukocyte Common Antigens Molecular Sequence Data RNA Splicing/genetics RNA, Messenger/analysis T-Lymphocytes/analysis Transfection
Chemicals
Antigens, Differentiation Histocompatibility Antigens RNA, Messenger Leukocyte Common Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsai A Y
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Streuli M
Saito H
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.10.4550-4555.1989
Published
1989-10-00
Pages
4550-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362542
Subset
IM
Grants
NIAID NIH HHS · AI-26598 · United States
NIDCR NIH HHS · DE07010 · United States
Databases
GENBANK
M29253
External Links
PubMed source DOI: 10.1128/mcb.9.10.4550-4555.1989 PMC Full Text View journal details
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