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PMID: 2527181 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Premeiotic change of nucleolus organizer size in Neurospora.

Genetics ·Vol. 122 ·No. 4 ·1989-08-00 ·Pages 783-91

Butler DK, Metzenberg RL

Abstract

We have investigated the heritability of nucleolus organizer region (NOR) size in Neurospora crassa. By pulsed-field gel electrophoresis, we followed in genetic crosses the size of the normal or "terminal" NORs and the size of a small interstitial NOR. Tetrad analysis revealed that changes in NOR size occur frequently in the sexual phase. Moreover, most size changes occurred in the period between fertilization and meiosis, although some changes occurred during and after meiosis. Unexpectedly, increases and decreases in NOR size were not equally frequent: decreases were more common. The NOR size changes generated during meiosis were not the result of unequal crossing over between NORs on homologous chromosomes.

MeSH Terms
Autoradiography Crosses, Genetic DNA, Fungal/genetics DNA, Ribosomal/genetics Electrophoresis Fertilization Meiosis Multigene Family Neurospora/genetics Neurospora crassa/genetics,physiology,ultrastructure Nucleolus Organizer Region/physiology,ultrastructure RNA, Fungal/genetics RNA, Ribosomal/genetics Sister Chromatid Exchange
Chemicals
DNA, Fungal DNA, Ribosomal RNA, Fungal RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Butler D K
Department of Physiological Chemistry, University of Wisconsin, Madison 53706.
Metzenberg R L
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/122.4.783
Published
1989-08-00
Pages
783-91
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203754
Subset
IM
Grants
NIGMS NIH HHS · GM 08995 · United States
External Links
PubMed source DOI: 10.1093/genetics/122.4.783 PMC Full Text View journal details
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