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PMID: 2524838 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of cDNA encoding Sm autoantigen: derivation of a cDNA for a B polypeptide of the U series of small nuclear ribonucleoprotein particles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 11 ·1989-06-00 ·Pages 4249-53

Ohosone Y, Mimori T, Griffith A, Akizuki M, Homma M, Craft J, Hardin JA

Abstract

The Sm snRNPs play a central role in the processing of pre-mRNA. Anti-Sm antibodies, the diagnostic hallmark of systemic lupus erythematosus, target the B'/B and D polypeptides of these snRNPs. We have used patient autoantibodies to clone a cDNA from a human fibroblast cDNA library that encodes the full length of a polypeptide identical with, or closely related to, polypeptide B. This cDNA is comprised of 1139 bases and contains an open reading frame of 855 nucleotides that is capable of encoding 285 amino acids. The first 223 amino acids at the NH2 terminus exhibit nearly complete homology with polypeptide N, a newly recognized brain- and heart-specific component of Sm snRNPs. The derived amino acid sequence for B differs from that of the N polypeptide primarily by a 50-amino acid insert 12 residues upstream from the homologous COOH termini of these polypeptides. The structural differences in these cDNAs for B and N may regulate tissue-specific alternative splicing mechanisms for mRNA. In addition, these clones make it possible to map in fine detail the most characteristic autoimmune responses of systemic lupus erythematosus.

MeSH Terms
Amino Acid Sequence Autoantigens/genetics,isolation & purification Base Sequence Cloning, Molecular DNA, Neoplasm/genetics HeLa Cells/immunology Humans Molecular Sequence Data RNA Precursors/genetics Recombinant Fusion Proteins/isolation & purification Restriction Mapping Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Thyroid Neoplasms/genetics,immunology snRNP Core Proteins
Chemicals
Autoantigens DNA, Neoplasm RNA Precursors Recombinant Fusion Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ohosone Y
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
Mimori T
Griffith A
Akizuki M
Homma M
Craft J
Hardin J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.11.4249
Published
1989-06-00
Pages
4249-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287428
Subset
IM
Grants
NIAID NIH HHS · AI 26853 · United States
NIADDK NIH HHS · AM 07107 · United States
NIAMS NIH HHS · AR 32549 · United States
Databases
GENBANK
J04564
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1073/pnas.86.11.4249 PMC Full Text View journal details
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