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PMID: 2519113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Maize pyruvate decarboxylase mRNA is induced anaerobically.

Plant molecular biology ·Vol. 13 ·No. 2 ·1989-08-00 ·Pages 213-22

Kelley PM

Abstract

A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

MeSH Terms
Amino Acid Sequence Anaerobiosis Base Sequence DNA/genetics DNA Probes DNA, Fungal/genetics Enzyme Induction Molecular Sequence Data Plants/enzymology,genetics Pyruvate Decarboxylase/genetics RNA, Messenger/biosynthesis,genetics Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid Zea mays/enzymology,genetics
Chemicals
DNA Probes DNA, Fungal RNA, Messenger DNA Pyruvate Decarboxylase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kelley P M
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
References (28)
28 references, click to expand
  1. Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5610-4 PMID: 3399506
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
    Anal Biochem. 1984 Jan;136(1):175-9 PMID: 6424501
  4. Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco.
    EMBO J. 1987 Jan;6(1):11-6 PMID: 15981329
  5. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  6. Induction of lactate dehydrogenase isozymes by oxygen deficit in barley root tissue.
    Plant Physiol. 1986 Nov;82(3):658-63 PMID: 16665087
  7. Regulation of Cytoplasmic and Vacuolar pH in Maize Root Tips under Different Experimental Conditions.
    Plant Physiol. 1982 Jun;69(6):1344-7 PMID: 16662399
  8. The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein.
    Mol Gen Genet. 1986 Dec;205(3):461-8 PMID: 2436026
  9. Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize.
    J Biol Chem. 1985 Apr 25;260(8):5050-4 PMID: 2580829
  10. Alcohol Dehydrogenase and Pyruvate Decarboxylase Activity in Leaves and Roots of Eastern Cottonwood (Populus deltoides Bartr.) and Soybean (Glycine max L.).
    Plant Physiol. 1987 Aug;84(4):1210-3 PMID: 16665586
  11. Simultaneous induction by anaerobiosis or 2,4-D of multiple enzymes specificed by two unlinked genes: differential Adh1-Adh2 expression in maize.
    Mol Gen Genet. 1973 Dec 31;127(3):215-27 PMID: 4782689
  12. The anaerobic proteins of maize.
    Cell. 1980 Jul;20(3):761-7 PMID: 7418006
  13. The complete amino Acid sequence for the anaerobically induced aldolase from maize derived from cDNA clones.
    Plant Physiol. 1986 Dec;82(4):1076-80 PMID: 16665137
  14. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  15. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1986 Nov 25;14(22):8963-77 PMID: 3537965
  16. The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level.
    Mol Gen Genet. 1983;192(1-2):247-52 PMID: 6358796
  17. Anaerobic expression of maize fructose-1,6-diphosphate aldolase.
    J Biol Chem. 1984 Nov 25;259(22):14180-3 PMID: 6501292
  18. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  19. Anaerobic expression of maize glucose phosphate isomerase I.
    J Biol Chem. 1984 Jan 10;259(1):673-7 PMID: 6706959
  20. Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli.
    J Bacteriol. 1987 Mar;169(3):1024-8 PMID: 3546263
  21. Molecular identification and isolation of the Waxy locus in maize.
    Cell. 1983 Nov;35(1):225-33 PMID: 6313224
  22. Patterns of polypeptide synthesis in various maize organs under anaerobiosis.
    Planta. 1980 Nov;150(1):89-94 PMID: 24306530
  23. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  24. Pyruvate Decarboxylase from Zea mays L. : I. Purification and Partial Characterization from Mature Kernels and Anaerobically Treated Roots.
    Plant Physiol. 1985 Sep;79(1):242-7 PMID: 16664379
  25. Anaerobically regulated aldolase gene of maize. A chimaeric origin?
    J Mol Biol. 1988 Aug 20;202(4):759-67 PMID: 3172237
  26. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase.
    J Bacteriol. 1987 Mar;169(3):949-54 PMID: 3029037
  27. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  28. Improved purification of pyruvate decarboxylase from wheat germ. Its partial characterisation and comparison with the yeast enzyme.
    Eur J Biochem. 1987 Aug 17;167(1):149-54 PMID: 3305013
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016139
Published
1989-08-00
Pages
213-22
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00016139 View journal details
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