Home LiteratureArticle Details
PMID: 3537965 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 14 ·No. 22 ·1986-11-25 ·Pages 8963-77

Kellermann E, Seeboth PG, Hollenberg CP

Abstract

The PDC1 gene of Saccharomyces cerevisiae, encoding pyruvate decarboxylase was sequenced. The gene contains an open reading frame of 1647 base pairs. The codon usage shows the same strong bias as found for some other glycolytic enzymes. Transcription starts mainly at -30 and terminates 100 base pairs downstream of the termination codon. In some strains a second termination site, 46 base pairs upstream of the stop codon was observed. The function of the promoter region was analyzed by fusion to the bacterial structural gene encoding beta-lactamase (bla). On multicopy plasmid or integrated in the genome, the expression of the bla gene showed the regulation of the authentic PDC1 gene.

MeSH Terms
Amino Acid Sequence Base Sequence Carboxy-Lyases/genetics Codon Genes Genes, Viral Promoter Regions, Genetic Pyruvate Decarboxylase/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Codon RNA, Messenger Carboxy-Lyases Pyruvate Decarboxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kellermann E
Seeboth P G
Hollenberg C P
References (23)
23 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Proteolysis in eukaryotic cells. Identification of multiple proteolytic enzymes in yeast.
    J Biol Chem. 1984 Nov 10;259(21):13334-43 PMID: 6149221
  3. Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.
    Biochim Biophys Acta. 1975 Oct 20;405(2):492-9 PMID: 1101964
  4. Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40.
    J Mol Biol. 1978 Dec 25;126(4):813-46 PMID: 218021
  5. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  7. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  8. Isolation and characterization of further cis- and trans-acting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Nov;176(3):427-31 PMID: 392242
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  11. Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4466-70 PMID: 7027263
  12. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  13. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  14. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  15. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1982 Apr 24;10(8):2625-37 PMID: 6281737
  16. Structure-function relationships in pyruvate decarboxylase of yeast and wheat germ.
    Ann N Y Acad Sci. 1982;378:287-305 PMID: 6805384
  17. Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis.
    J Bacteriol. 1982 Sep;151(3):1146-52 PMID: 7050079
  18. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  19. Transcription of the bacterial beta-lactamase gene in Saccharomyces cerevisiae.
    Gene. 1982 Nov;20(1):1-10 PMID: 6761237
  20. Genetic applications of yeast transformation with linear and gapped plasmids.
    Methods Enzymol. 1983;101:228-45 PMID: 6310326
  21. The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.
    J Bacteriol. 1983 Nov;156(2):625-35 PMID: 6313610
  22. The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level.
    Mol Gen Genet. 1983;192(1-2):247-52 PMID: 6358796
  23. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.22.8963
Published
1986-11-25
Pages
8963-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311923
Subset
IM
Databases
GENBANK
X04675
External Links
PubMed source DOI: 10.1093/nar/14.22.8963 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2