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PMID: 2511422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Autoregulation of the ccd operon in the F plasmid.

Molecular & general genetics : MGG ·Vol. 218 ·No. 3 ·1989-09-00 ·Pages 481-6

de Feyter R, Wallace C, Lane D

Abstract

Mini-F sequences, including the promoter and portions of the ccd region, were inserted upstream of lacZ in promoterless lacZ vectors, and beta-galactosidase specific activities were measured. The results showed that the H (ccdA), G (ccdB) and D genes, together with a promoter, comprise an operon. Ccd operon expression was shown to be regulated at the level of transcription by the G gene product, probably in concert with the H gene product. Thus expression is autoregulated. Expression of the D gene was largely dependent on the ccd promoter, although low levels of transcription from another promoter within the ccd coding region were detected.

MeSH Terms
Escherichia coli/genetics F Factor Gene Expression Regulation Operon Promoter Regions, Genetic Transcription, Genetic beta-Galactosidase/metabolism
Chemicals
beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
de Feyter R
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Wallace C
Lane D
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332413
Published
1989-09-00
Pages
481-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332413
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