Improved vectors and genome-wide libraries for CRISPR scree…

Abstract

暂无摘要

MeSH Terms

Animals Clustered Regularly Interspaced Short Palindromic Repeats Genetic Vectors Genome Humans Mice Models, Genetic RNA Interference

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sanjana Neville E

Broad Institute of MIT and Harvard 7 Cambridge Center Cambridge, MA 02142, USA. | McGovern Institute for Brain Research Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Brain and Cognitive Sciences Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139, USA.

Shalem Ophir

Broad Institute of MIT and Harvard 7 Cambridge Center Cambridge, MA 02142, USA. | McGovern Institute for Brain Research Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Brain and Cognitive Sciences Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139, USA.

Zhang Feng

Broad Institute of MIT and Harvard 7 Cambridge Center Cambridge, MA 02142, USA. | McGovern Institute for Brain Research Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Brain and Cognitive Sciences Massachusetts Institute of Technology Cambridge, MA 02139, USA. | Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139, USA.

References (6)

6 references, click to expand

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells.
Nature. 2014 May 22;509(7501):487-91 PMID: 24717434
Genome-scale CRISPR-Cas9 knockout screening in human cells.
Science. 2014 Jan 3;343(6166):84-87 PMID: 24336571
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library.
Nat Biotechnol. 2014 Mar;32(3):267-73 PMID: 24535568
DNA targeting specificity of RNA-guided Cas9 nucleases.
Nat Biotechnol. 2013 Sep;31(9):827-32 PMID: 23873081
Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.
Sci Rep. 2014 Feb 03;4:3943 PMID: 24487629
Genetic screens in human cells using the CRISPR-Cas9 system.
Science. 2014 Jan 3;343(6166):80-4 PMID: 24336569

Improved vectors and genome-wide libraries for CRISPR screening.

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