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PMID: 2506426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts.

Molecular & general genetics : MGG ·Vol. 218 ·No. 2 ·1989-08-00 ·Pages 289-92

Galili S, Fromm H, Aviv D, Edelman M, Galun E

Abstract

A streptomycin resistant Nicotiana plastome mutant, X/strR6, was subjected to molecular analysis. In this mutant, a single nucleotide transition, C----T, in the chloroplast gene for ribosomal protein S12 alters codon 90 from proline to serine while the nucleotide sequence of the chloroplast 16 S rRNA gene is identical to that of the wild type. Mutant X/strR6 thus differs from several previously reported streptomycin resistant chloroplast mutants which are altered in the gene for 16 S rRNA.

MeSH Terms
Base Sequence Chloroplasts/drug effects Cloning, Molecular Codon Drug Resistance/genetics Exons Mutation Plant Proteins/genetics Plants, Toxic Proline/genetics Ribosomal Proteins/genetics Serine/genetics Streptomycin/pharmacology Tobacco/drug effects,genetics
Chemicals
Codon Plant Proteins Ribosomal Proteins ribosomal protein S12 Serine Proline Streptomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Galili S
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Fromm H
Aviv D
Edelman M
Galun E
References (19)
19 references, click to expand
  1. Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts.
    Cell. 1987 Jan 16;48(1):111-9 PMID: 3791410
  2. MENDELIAN AND NON-MENDELIAN INHERITANCE OF STREPTOMYCIN RESISTANCE IN CHLAMYDOMONAS REINHARDI.
    Proc Natl Acad Sci U S A. 1954 May;40(5):356-63 PMID: 16589487
  3. Efficient induction and selection of chloroplast-encoded antibiotic-resistant mutants in Nicotiana.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1485-9 PMID: 16593549
  4. The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana.
    Nucleic Acids Res. 1986 Jan 24;14(2):883-98 PMID: 3945556
  5. Trans-splicing in chloroplasts: the rps 12 loci of Nicotiana tabacum.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):372-6 PMID: 3422433
  6. Nucleotide sequence, promoter analysis, and linkage mapping of the unusually organized operon encoding ribosomal proteins S7 and S12 in maize chloroplast.
    J Biol Chem. 1987 Nov 5;262(31):15251-5 PMID: 2822717
  7. The 5' part of the gene for ribosomal protein S12 is located 30 kbp downstream from its 3' part in tobacco chloroplast genome.
    Nucleic Acids Res. 1986 Apr 11;14(7):3143 PMID: 3960739
  8. The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts.
    EMBO J. 1987 Nov;6(11):3233-7 PMID: 16453804
  9. E. coli ribosomes with a C912 to U base change in the 16S rRNA are streptomycin resistant.
    EMBO J. 1986 Dec 20;5(13):3705-8 PMID: 3104030
  10. Mechanism of streptomycin action on bacteria: a unitary hypothesis.
    Nature. 1961 Nov 18;192:633-7 PMID: 13915908
  11. On the nature of antibiotic binding sites in ribosomes.
    Biochimie. 1987 Aug;69(8):863-9 PMID: 3122848
  12. The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis.
    Nucleic Acids Res. 1984 Mar 26;12(6):2851-9 PMID: 6324129
  13. Non-Mendelian streptomycin-resistant tobacco mutant with altered chlorplasts and mitochondria.
    Nature. 1975 May 29;255(5507):401-2 PMID: 1128700
  14. Clone bank of Nicotiana tabacum chloroplast DNA: mapping of the alpha, beta and epsilon subunits of the ATPase coupling factor, the large subunit of ribulosebisphosphate carboxylase, and the 32-kDal membrane protein.
    Gene. 1983 Nov;25(2-3):271-80 PMID: 6229453
  15. Complete sequence of 'divided' rps12 (r-protein S12) and rps7 (r-protein S7) gene in soybean chloroplast DNA.
    Nucleic Acids Res. 1987 Mar 11;15(5):2387 PMID: 3562230
  16. Streptomycin-resistance of Euglena gracilis chloroplasts: identification of a point mutation in the 16S rRNA gene in an invariant position.
    Nucleic Acids Res. 1985 Jun 25;13(12):4299-310 PMID: 3925438
  17. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli.
    Nature. 1969 Apr 26;222(5191):333-9 PMID: 4181187
  18. A novel site for streptomycin resistance in the "530 loop" of chloroplast 16S ribosomal RNA.
    Plant Mol Biol. 1989 May;12(5):499-505 PMID: 24271066
  19. STREPTOMYCIN, SUPPRESSION, AND THE CODE.
    Proc Natl Acad Sci U S A. 1964 May;51:883-90 PMID: 14173007
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331280
Published
1989-08-00
Pages
289-92
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331280
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