Home LiteratureArticle Details
PMID: 2496143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tumor necrosis factor and immune interferon synergistically induce cytochrome b-245 heavy-chain gene expression and nicotinamide-adenine dinucleotide phosphate hydrogenase oxidase in human leukemic myeloid cells.

The Journal of clinical investigation ·Vol. 83 ·No. 5 ·1989-05-00 ·Pages 1570-9

Cassatella MA, Hartman L, Perussia B, Trinchieri G

Abstract

Recombinant tumor necrosis factor (rTNF) and rIFN-gamma induce in the human leukemia cell lines HL-60, ML3, and U937 the accumulation of transcripts of the X chromosome-linked chronic granulomatous disease (X-CGD) gene, encoding the 91-kD heavy chain of cytochrome b-245, a component of the NADPH oxidase of phagocytic cells. The gene is induced within 6 h by either cytokine, and its accumulation is observed upon induction with rIFN-gamma up to 5 d. The combined effect of the two cytokines is more than additive. rIFN-gamma also induces accumulation of X-CGD mRNA in immature myeloid cells from peripheral blood of chronic myeloid leukemia (CML) patients, whereas rTNF has almost no effect. The cells from CML patients constitutively express TNF mRNA, suggesting that endogenously produced TNF may play a role in the effect of rIFN-gamma on these cells. rTNF induces X-CGD gene expression in the myeloid cell lines acting, at least in part, at the transcriptional level, as shown in nuclear run-on experiments. The gene encoding the 22-kD light chain of cytochrome b-245 is constitutively expressed in the human myeloid cell lines and the accumulation of its transcripts is affected by neither rTNF nor rIFN-gamma, rTNF and rIFN-gamma synergistically to induce the cell lines to express the cytochrome b-245 heterodimer (as evaluated by its visible spectrum), and to produce NADPH oxidase activity and H2O2 upon stimulation with phorbol diesters.

MeSH Terms
Cell Line Cytochrome b Group/genetics Drug Synergism Gene Expression Regulation/drug effects Granulomatous Disease, Chronic/enzymology,genetics Humans Interferon-gamma/pharmacology Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics Leukemia, Myeloid/enzymology,genetics,metabolism NADH, NADPH Oxidoreductases/metabolism NADPH Oxidases Oxygen Consumption/drug effects Protein Synthesis Inhibitors/pharmacology Recombinant Proteins Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Cytochrome b Group Protein Synthesis Inhibitors Recombinant Proteins Tumor Necrosis Factor-alpha cytochrome b245 Interferon-gamma NADH, NADPH Oxidoreductases NADPH Oxidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cassatella M A
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Hartman L
Perussia B
Trinchieri G
References (53)
53 references, click to expand
  1. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.
    J Cell Biol. 1967 Feb;32(2):415-38 PMID: 10976232
  2. Expression of the X-CGD gene during induced differentiation of myeloid leukemia cell line HL-60.
    Mol Cell Biol. 1988 Jul;8(7):2804-10 PMID: 3165493
  3. Evaluation of the Bradford method for protein determination in body fluids.
    J Clin Chem Clin Biochem. 1978 Sep;16(9):533-4 PMID: 712344
  4. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
    Blood. 1979 Sep;54(3):713-33 PMID: 288488
  5. Altered pattern of differentiation and proliferation of HL-60 promyelocytic leukemia cells in the presence of leucocyte conditioned medium.
    Leuk Res. 1980;4(3):301-7 PMID: 6931962
  6. Molecular alterations in the respiratory chain of rat liver after chronic ethanol consumption.
    J Biol Chem. 1981 Jun 25;256(12):6090-7 PMID: 6263903
  7. Cytochrome b-245 of neutrophils is also present in human monocytes, macrophages and eosinophils.
    Biochem J. 1981 Apr 15;196(1):363-7 PMID: 7306076
  8. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity.
    J Exp Med. 1982 Dec 1;156(6):1780-93 PMID: 6816896
  9. Correlation between differentiation, expression of monocyte-specific antigens, and cytotoxic functions in human promyelocytic cell lines treated with leukocyte-conditioned medium.
    J Immunol. 1983 Mar;130(3):1120-8 PMID: 6185572
  10. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1.
    J Immunol. 1983 May;130(5):2133-41 PMID: 6833758
  11. Development of cytochrome b and an active oxidase system in association with maturation of a human promyelocytic (HL-60) cell line.
    J Cell Biol. 1982 Dec;95(3):720-6 PMID: 6296156
  12. Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):472-6 PMID: 6188151
  13. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity.
    J Exp Med. 1983 Sep 1;158(3):670-89 PMID: 6411853
  14. The measurement of superoxide anion production by granulocytes In whole blood. A clinical test for the evaluation of phagocyte function and serum opsonic capacity.
    Eur J Clin Invest. 1983 Aug;13(4):363-8 PMID: 6311564
  15. Immune interferon induces the receptor for monomeric IgG1 on human monocytic and myeloid cells.
    J Exp Med. 1983 Oct 1;158(4):1092-113 PMID: 6225822
  16. Immune interferon and leukocyte-conditioned medium induce normal and leukemic myeloid cells to differentiate along the monocytic pathway.
    J Exp Med. 1983 Dec 1;158(6):2058-80 PMID: 6417261
  17. Development of the superoxide-generating system during differentiation of the HL-60 human promyelocytic leukemia cell line.
    J Biol Chem. 1984 Mar 25;259(6):3771-6 PMID: 6323439
  18. Lineage infidelity of a human myelogenous leukemia cell line.
    Blood. 1984 Nov;64(5):1059-63 PMID: 6091813
  19. Cytochrome b translocation to human neutrophil plasma membranes and superoxide release. Differential effects of N-formylmethionylleucylphenylalanine, phorbol myristate acetate, and A23187.
    J Biol Chem. 1985 Feb 25;260(4):2409-14 PMID: 2982817
  20. Regulation of the oxidative response of human granulocytes to chemoattractants. No evidence for stimulated traffic of redox enzymes between endo and plasma membranes.
    J Biol Chem. 1985 Jun 10;260(11):6541-7 PMID: 2987226
  21. Role of immune interferon in the monocytic differentiation of human promyelocytic cell lines induced by leukocyte conditioned medium.
    Blood. 1985 Sep;66(3):583-94 PMID: 3927999
  22. Partial purification of the superoxide-generating system of macrophages. Possible association of the NADPH oxidase activity with a low-potential (-247 mV) cytochrome b.
    Biochim Biophys Acta. 1985 Nov 27;810(2):164-73 PMID: 4063352
  23. Activation by gamma interferon of human macrophage capability to produce toxic oxygen molecules is accompanied by decreased Km of the superoxide-generating NADPH oxidase.
    Biochem Biophys Res Commun. 1985 Nov 15;132(3):908-14 PMID: 3000367
  24. Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):446-50 PMID: 3455781
  25. Molecular basis of macrophage activation. Expression of the low potential cytochrome b and its reduction upon cell stimulation in activated macrophages.
    J Immunol. 1986 Feb 15;136(4):1393-9 PMID: 3003196
  26. DNA linkage analysis of X chromosome-linked chronic granulomatous disease.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3398-401 PMID: 3010296
  27. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location.
    Nature. 1986 Jul 3-9;322(6074):32-8 PMID: 2425263
  28. Gamma interferon is able to enhance the oxidative metabolism of human neutrophils.
    Biochem Biophys Res Commun. 1986 Aug 14;138(3):1276-82 PMID: 3092821
  29. Measurement of NADPH oxidase activity in detergent lysates of human and mouse macrophage monolayers.
    J Immunol Methods. 1986 Sep 27;92(2):231-40 PMID: 3760584
  30. Tumor necrosis factor and lymphotoxin induce differentiation of human myeloid cell lines in synergy with immune interferon.
    J Exp Med. 1986 Oct 1;164(4):1206-25 PMID: 3093627
  31. Identity of differentiation inducing factor and tumour necrosis factor.
    Nature. 1986 Sep 25-Oct 1;323(6086):338-40 PMID: 3463866
  32. The O2- -forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function.
    Biochim Biophys Acta. 1986 Nov 4;853(1):65-89 PMID: 3021215
  33. Tumor necrosis factor reduces c-myc expression and cooperates with interferon-gamma in HeLa cells.
    Science. 1986 Dec 12;234(4782):1419-21 PMID: 3097823
  34. Tumor necrosis factor inhibits MYC expression in HL-60 cells at the level of mRNA transcription.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):469-73 PMID: 3099296
  35. Immune interferon enhances functional properties of human granulocytes: role of Fc receptors and effect of lymphotoxin, tumor necrosis factor, and granulocyte-macrophage colony-stimulating factor.
    J Immunol. 1987 Feb 1;138(3):765-74 PMID: 3100615
  36. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease.
    Nature. 1987 Mar 5-11;326(6108):88-91 PMID: 3821877
  37. Recombinant human tumor necrosis factor alpha regulates c-myc expression in HL-60 cells at the level of transcription.
    Blood. 1987 Jul;70(1):200-5 PMID: 3474039
  38. Induction and regulation of mRNA encoding 26-kDa protein in human cell lines treated with recombinant human tumor necrosis factor.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4557-61 PMID: 3496595
  39. Cell-free activation of phagocyte NADPH-oxidase: tissue and differentiation-specific expression of cytosolic cofactor activity.
    Biochem Biophys Res Commun. 1987 Jun 30;145(3):1198-204 PMID: 3038092
  40. Tumor necrosis factor and interleukin-1 cause a rapid and transient stimulation of c-fos and c-myc mRNA levels in human fibroblasts.
    J Biol Chem. 1987 Sep 5;262(25):11908-11 PMID: 3497923
  41. Recombinant interferon gamma augments phagocyte superoxide production and X-chronic granulomatous disease gene expression in X-linked variant chronic granulomatous disease.
    J Clin Invest. 1987 Oct;80(4):1009-16 PMID: 2821069
  42. The quaternary structure of the plasma membrane b-type cytochrome of human granulocytes.
    Biochim Biophys Acta. 1988 Jan 20;932(1):71-83 PMID: 3337799
  43. Transcriptional regulation of the interferon-beta 2/B cell differentiation factor BSF-2/hepatocyte-stimulating factor gene in human fibroblasts by other cytokines.
    J Immunol. 1988 Feb 1;140(3):974-7 PMID: 3276786
  44. Induction of synthesis of components of the hydrogen peroxide-generating oxidase during activation of the human monocytic cell line U937 by interferon-gamma.
    Biochem J. 1987 Nov 15;248(1):281-3 PMID: 3124810
  45. Certain lymphoid cells contain the membrane-associated component of the phagocyte-specific NADPH oxidase.
    J Immunol. 1988 Mar 1;140(5):1611-7 PMID: 2831270
  46. Interaction of Fc receptor (CD16) ligands induces transcription of interleukin 2 receptor (CD25) and lymphokine genes and expression of their products in human natural killer cells.
    J Exp Med. 1988 Feb 1;167(2):452-72 PMID: 2831292
  47. Specific activation by concanavalin A of the superoxide anion generation capacity during U937 differentiation.
    Biochem Biophys Res Commun. 1988 Mar 15;151(2):802-8 PMID: 2831904
  48. Interferon-gamma activates human neutrophil oxygen metabolism and exocytosis.
    Immunology. 1988 Mar;63(3):499-506 PMID: 2832315
  49. Primary structure and unique expression of the 22-kilodalton light chain of human neutrophil cytochrome b.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3319-23 PMID: 3368442
  50. Partial correction of the phagocyte defect in patients with X-linked chronic granulomatous disease by subcutaneous interferon gamma.
    N Engl J Med. 1988 Jul 21;319(3):146-51 PMID: 2838754
  51. The respiratory burst oxidase.
    Hematol Oncol Clin North Am. 1988 Jun;2(2):201-12 PMID: 2839456
  52. Induction of phagocyte cytochrome b heavy chain gene expression by interferon gamma.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):5215-9 PMID: 2839835
  53. Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
    Int J Cancer. 1976 May 15;17(5):565-77 PMID: 178611
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114054
Published
1989-05-00
Pages
1570-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303863
Subset
IM
Grants
NCI NIH HHS · CA-10815 · United States
NCI NIH HHS · CA-20833 · United States
NCI NIH HHS · CA-32898 · United States
External Links
PubMed source DOI: 10.1172/JCI114054 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2