Home LiteratureArticle Details
PMID: 2495523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of DNA-binding proteins with the tissue-specific human apolipoprotein-AII enhancer.

Nucleic acids research ·Vol. 17 ·No. 6 ·1989-03-25 ·Pages 2283-300

Lucero MA, Sanchez D, Ochoa AR, Brunel F, Cohen GN, Baralle FE, Zakin MM

Abstract

The identification of the binding sites for liver nuclear proteins present in the enhancer that control the cell specific transcription of the human apolipoprotein AII gene is reported. Five adjacent binding sites (motifs I to V) were identified. The motifs III, IV and V can be occupied differently by liver or HeLa nuclear proteins. Two hypersensitive zones (between motifs II-III and IV-V) are present only when liver nuclear extracts were tested. A first characterization of the factors reveal that motif IV interacts with the hepatic transcription factors Tf-LF1 (29) and LF-A1 (28, 30). A CCAAT binding protein, different from CTF/NF1, appears to bind to the motif II. The different binding sites share specific DNA sequences principally with 5' regulatory regions of other apolipoprotein genes.

MeSH Terms
Apolipoprotein A-II Apolipoproteins A/genetics Base Sequence Binding Sites Cell Nucleus/analysis DNA-Binding Proteins/metabolism Deoxyribonuclease I Enhancer Elements, Genetic HeLa Cells Humans Liver/ultrastructure Molecular Sequence Data Nuclear Proteins/metabolism Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription Factors/metabolism
Chemicals
Apolipoprotein A-II Apolipoproteins A DNA-Binding Proteins Nuclear Proteins Transcription Factors Deoxyribonuclease I
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lucero M A
Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
Sanchez D
Ochoa A R
Brunel F
Cohen G N
Baralle F E
Zakin M M
References (37)
37 references, click to expand
  1. The plasma lecithins:cholesterol acyltransferase reaction.
    J Lipid Res. 1968 Mar;9(2):155-67 PMID: 4868699
  2. Cell type-specific expression of the human apoB gene is controlled by two cis-acting regulatory regions.
    J Biol Chem. 1988 Aug 15;263(23):11452-8 PMID: 3165376
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. Effect of apolipoprotein A-II on the structure of high-density lipoproteins: relationship to the activity of lecithin:cholesterol acyl transferase in vitro.
    Ann N Y Acad Sci. 1980;348:160-73 PMID: 6772075
  5. Transport of apolipoproteins A-I and A-II by human thoracic duct lymph.
    J Clin Invest. 1981 Mar;67(3):857-66 PMID: 7204560
  6. The effects of subfractions of high density lipoprotein on cholesterol efflux from cultured fibroblasts. Regulation of low density lipoprotein receptor activity.
    J Biol Chem. 1981 Aug 25;256(16):8348-56 PMID: 6267041
  7. Multivariate analyses of serum apolipoproteins and risk factors in relation to acute myocardial infarction.
    Arteriosclerosis. 1981 Jul-Aug;1(4):273-9 PMID: 7295199
  8. In vitro activation of the enzymic activity of hepatic lipase by apoA-II.
    FEBS Lett. 1981 Aug 31;131(2):366-8 PMID: 6795058
  9. On the mechanism of the displacement of apolipoprotein A-I by apolipoprotein A-II from the high density lipoprotein surface. Effect of concentration and molecular forms of apolipoprotein A-II.
    J Biol Chem. 1982 Jun 25;257(12):7189-95 PMID: 6806266
  10. Distribution of high density lipoprotein particles with different apoprotein composition: particles with A-I and A-II and particles with A-I but no A-II.
    J Lipid Res. 1982 Jul;23(5):747-53 PMID: 6811682
  11. Mechanism of inhibition of hepatic triglyceride lipase from human postheparin plasma by apolipoproteins A-I and A-II.
    J Biochem. 1982 Sep;92(3):865-70 PMID: 6815170
  12. Effect of apolipoproteins on the hepatic lipase-catalyzed hydrolysis of human plasma high density lipoprotein2-triacylglycerols.
    Biochim Biophys Acta. 1982 Nov 12;713(2):292-9 PMID: 7150617
  13. Risk factors for peripheral atherosclerosis. Retrospective evaluation by stepwise discriminant analysis.
    Arteriosclerosis. 1983 Jan-Feb;3(1):57-63 PMID: 6401992
  14. Intestinal synthesis, secretion, and transport of lipoproteins.
    Annu Rev Physiol. 1983;45:625-36 PMID: 6405679
  15. Biosynthesis of human preproapolipoprotein A-II.
    J Biol Chem. 1983 Nov 25;258(22):14054-9 PMID: 6315718
  16. Cell type-specific transcriptional enhancement in vitro requires the presence of trans-acting factors.
    EMBO J. 1985 Nov;4(11):3005-13 PMID: 2998773
  17. The structure of the human apolipoprotein C-II gene. Electron microscopic analysis of RNA:DNA hybrids, complete nucleotide sequence, and identification of 5' homologous sequences among apolipoprotein genes.
    J Biol Chem. 1985 Dec 5;260(28):15211-21 PMID: 2415514
  18. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  19. Organ distribution of apolipoprotein gene transcripts in 6-12 week postfertilization human embryos.
    J Embryol Exp Morphol. 1986 Sep;97:177-87 PMID: 2432145
  20. Dual tissue-specific expression of apo-AII is directed by an upstream enhancer.
    Nucleic Acids Res. 1987 May 11;15(9):3801-21 PMID: 3035501
  21. Structure and expression of the human apolipoprotein A-IV gene.
    J Biol Chem. 1987 Jun 15;262(17):7973-81 PMID: 3036793
  22. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen.
    Cell. 1987 Sep 11;50(6):847-61 PMID: 3040262
  23. Two different liver-specific factors stimulate in vitro transcription from the human alpha 1-antitrypsin promoter.
    EMBO J. 1988 Jul;7(7):2075-87 PMID: 3262058
  24. Identification and characterization of transcriptional regulatory regions associated with expression of the human apolipoprotein E gene.
    J Biol Chem. 1988 Sep 15;263(26):13340-9 PMID: 3166458
  25. Two distinct factors interact with the promoter regions of several liver-specific genes.
    EMBO J. 1988 Jun;7(6):1711-9 PMID: 2844524
  26. Different liver nuclear proteins binds to similar DNA sequences in the 5' flanking regions of three hepatic genes.
    Nucleic Acids Res. 1989 Jan 11;17(1):119-33 PMID: 2911463
  27. Evolution of apolipoprotein E: mouse sequence and evidence for an 11-nucleotide ancestral unit.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8085-9 PMID: 3865219
  28. Sequence-specific interactions of nuclear factors with the insulin gene enhancer.
    Cell. 1986 Apr 11;45(1):35-44 PMID: 3006925
  29. Transcriptional selectivity of viral genes in mammalian cells.
    Cell. 1986 Sep 12;46(6):795-805 PMID: 3530495
  30. Properties of a CCAAT box-binding protein.
    Nucleic Acids Res. 1987 Sep 25;15(18):7265-82 PMID: 3477778
  31. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP.
    Cell. 1987 Oct 23;51(2):251-60 PMID: 2822255
  32. Several distinct "CCAAT" box binding proteins coexist in eukaryotic cells.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):757-61 PMID: 3422457
  33. Different cis-acting DNA elements control expression of the human apolipoprotein AI gene in different cell types.
    Mol Cell Biol. 1988 Feb;8(2):605-14 PMID: 2895420
  34. Human apolipoprotein CIII gene expression is regulated by positive and negative cis-acting elements and tissue-specific protein factors.
    J Biol Chem. 1988 May 15;263(14):6857-64 PMID: 2834395
  35. Expression of the human apolipoprotein E gene is regulated by multiple positive and negative elements.
    J Biol Chem. 1988 Jun 15;263(17):8300-8 PMID: 3372525
  36. Interactions of DNA-binding proteins with the 5' region of the human transferrin gene.
    J Biol Chem. 1988 Jul 25;263(21):10180-5 PMID: 2839477
  37. Apoprotein synthesis by human duodenojejunal mucosa.
    Gastroenterology. 1978 Oct;75(4):677-82 PMID: 361487
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.6.2283
Published
1989-03-25
Pages
2283-300
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317596
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.6.2283 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2