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PMID: 2494659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of an amyloid beta precursor protein that binds heparin and contains tyrosine sulfate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 6 ·1989-03-00 ·Pages 2066-9

Schubert D, LaCorbiere M, Saitoh T, Cole G

Abstract

A secreted form of the amyloid beta protein precursor was isolated from the growth conditioned medium of the PC12 sympathetic nerve-like cell line. This protein is recognized by an antiserum that detects a protein of 140 kDa and a less abundant species of 115 kDa on NaDodSO4/acrylamide gels. The amyloid precursor proteins contain O-linked sugars and tyrosine sulfate and bind to the glycosaminoglycan heparin. These results suggest a role for extracellular sulfated glycoproteins in the pathogenesis of Alzheimer disease.

MeSH Terms
Adrenal Gland Neoplasms/metabolism Alzheimer Disease Amyloid/analysis,metabolism Amyloid beta-Protein Precursor Animals Blotting, Western Carbohydrates/analysis Electrophoresis, Polyacrylamide Gel Heparin/metabolism Immunoassay Molecular Weight Peptide Fragments/analysis,metabolism Pheochromocytoma/metabolism Protease Inhibitors Protein Precursors/analysis,metabolism Rats Tumor Cells, Cultured Tyrosine/analogs & derivatives,analysis
Chemicals
Amyloid Amyloid beta-Protein Precursor Carbohydrates Peptide Fragments Protease Inhibitors Protein Precursors tyrosine O-sulfate Tyrosine Heparin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schubert D
Salk Institute for Biological Studies, San Diego, CA 92138.
LaCorbiere M
Saitoh T
Cole G
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.6.2066
Published
1989-03-00
Pages
2066-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286848
Subset
IM
Grants
NIA NIH HHS · AG 05131 · United States
NIA NIH HHS · F2 AG 05424A · United States
NINDS NIH HHS · NS 09658 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.6.2066 PMC Full Text View journal details
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