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PMID: 2480259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oxygen free radical scavengers protect rat islet cells from damage by cytokines.

Diabetologia ·Vol. 32 ·No. 11 ·1989-11-00 ·Pages 792-6

Sumoski W, Baquerizo H, Rabinovitch A

Abstract

A possible role for oxygen free radicals in mediating the cytotoxic effects of cytokines in islets was sought by the use of agents that scavenge free radicals. Rat islet cell monolayer cultures were incubated for 6 days with t-butylhydroperoxide, alloxan, streptozotocin, or the cytokines, interleukin 1, tumour necrosis factor, and interferon gamma, without and together with the oxygen free radical scavenger combination of dimethylthiourea and citiolone, and islet cell lysis was measured in a 51chromium cytotoxicity assay. The free radical scavengers significantly inhibited the islet cell cytotoxic effects of t-butylhydroperoxide and alloxan, but not streptozotocin. Similarly, the cytotoxic effects of the cytokine combinations of interleukin 1 plus tumour necrosis factor, interferon gamma plus tumour necrosis factor, and interferon gamma plus interleukin 1 were significantly inhibited by the free radical scavenger combination of dimethylthiourea and citiolone. These results suggest that the cytokine products of macrophages and lymphocytes infiltrating islets in Type 1 (insulin-dependent) diabetes may contribute to B-cell damage by inducing the production of oxygen free radicals in the islet cells.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Alloxan/pharmacology Animals Biological Factors/pharmacology Cell Survival/drug effects Cells, Cultured Cytokines Free Radicals Interferon-gamma/pharmacology Interleukin-1/pharmacology Islets of Langerhans/cytology,drug effects Peroxides/pharmacology Rats Rats, Inbred Strains Recombinant Proteins/pharmacology Streptozocin/pharmacology Thiophenes/pharmacology Thiourea/analogs & derivatives,pharmacology Tumor Necrosis Factor-alpha/pharmacology tert-Butylhydroperoxide
Chemicals
Biological Factors Cytokines Free Radicals Interleukin-1 Peroxides Recombinant Proteins Thiophenes Tumor Necrosis Factor-alpha Streptozocin Alloxan citiolone Interferon-gamma 1,3-dimethylthiourea tert-Butylhydroperoxide Thiourea 1-Methyl-3-isobutylxanthine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sumoski W
Department of Medicine, University of Alberta, Edmonton, Canada.
Baquerizo H
Rabinovitch A
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31 references, click to expand
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Article Info
Journal
Diabetologia
Abbr.
Diabetologia
ISSN
0012-186X
DOI
10.1007/BF00264909
Published
1989-11-00
Pages
792-6
Language
English
Region
Germany
NLM ID
0006777
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00264909 View journal details
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