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PMID: 2479028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 22 ·1989-11-00 ·Pages 8742-6

Pinto AL, Steitz JA

Abstract

HeLa cell nuclear extracts contain a protein reactive with antibodies against PRP8, a polypeptide essential for pre-mRNA splicing in yeast and a specific component of the yeast U5 small nuclear ribonucleoprotein (snRNP) [Lossky, M., Anderson, G. J., Jackson, S. P. & Beggs, J. (1987) Cell 51, 1019-1026]. The mammalian protein appears as a doublet at approximately 200 kDa, smaller than the 260-kDa yeast protein, and possesses an Sm epitope as determined by immunoblotting. Its association with a snRNP of the Sm class other than U1 or U2 is indicated by its immunoprecipitation by anti-Sm and anti-trimethylguanosine antibodies but not by anti-(U1) or anti-(U2) RNP sera. Gradient fractionation of splicing extracts demonstrates that the 200-kDa protein is a component of the U4/5/6 snRNP complex and of U5 snRNPs. It is also present in affinity-purified spliceosomes.

MeSH Terms
Antibodies, Monoclonal Cell Nucleus/analysis Centrifugation, Density Gradient Endoribonucleases Epitopes/analysis Fungal Proteins/analysis HeLa Cells/analysis Humans Molecular Weight RNA Splicing Ribonuclease H Ribonucleoproteins/analysis Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae/analysis
Chemicals
Antibodies, Monoclonal Epitopes Fungal Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear Endoribonucleases Ribonuclease H
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinto A L
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.
Steitz J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.22.8742
Published
1989-11-00
Pages
8742-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298364
Subset
IM
Grants
NIGMS NIH HHS · GM26154 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.22.8742 PMC Full Text View journal details
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