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PMID: 2475511 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cytokine-induced respiratory burst of human neutrophils: dependence on extracellular matrix proteins and CD11/CD18 integrins.

The Journal of cell biology ·Vol. 109 ·No. 3 ·1989-09-00 ·Pages 1341-9

Nathan C, Srimal S, Farber C, Sanchez E, Kabbash L, Asch A, Gailit J, Wright SD

Abstract

Human polymorphonuclear leukocytes (PMN) released large quantities of hydrogen peroxide in response to tumor necrosis factor, but only when the cells were adherent to surfaces coated with extracellular matrix proteins. The PMN did not respond when exposed to cytokines and matrix proteins in suspension, or when exposed to cytokines while adherent to surfaces coated with stearic acid. PMN from children with genetic deficiency of the CD11/CD18 integrins underwent a normal respiratory burst upon adherence to uncoated polystyrene, but not in response to tumor necrosis factor when tested on polystyrene that was coated with serum, fibronectin, vitronectin, fibrinogen, thrombospondin, or laminin. Anti-CD18 antibodies, alone of sixteen antibodies tested, induced a similar defect in PMN from normal donors, when the PMN were tested on surfaces coated with serum, fibrinogen, thrombospondin, or laminin; no defect was induced by the anti-CD18 monoclonal antibody IB4 in normal PMN tested on surfaces coated with fibronectin or vitronectin. Thus, for cytokines to induce a respiratory burst in PMN, the cells must be able to use CD11/CD18 integrins and must interact with matrix proteins in the solid phase. CD11/CD18, which is already known to serve as a receptor for fibrinogen, may also be a receptor for thrombospondin and laminin. Finally, receptor(s) exist on PMN for fibronectin and vitronectin which are not blocked by the anti-CD18 antibody IB4 but which are nonetheless CD11/CD18 dependent.

MeSH Terms
Antibodies Antibodies, Monoclonal Antigens, Differentiation/immunology Biological Factors/pharmacology Cell Adhesion Child Cytokines Extracellular Matrix/physiology Fibrinogen/physiology Fibronectins/physiology Glycoproteins/physiology Humans Hydrogen Peroxide/blood In Vitro Techniques Kinetics Lymphocyte Function-Associated Antigen-1 Membrane Glycoproteins/physiology Neutrophils/drug effects,physiology Tetradecanoylphorbol Acetate/pharmacology Thrombospondins Vitronectin
Chemicals
Antibodies Antibodies, Monoclonal Antigens, Differentiation Biological Factors Cytokines Fibronectins Glycoproteins Lymphocyte Function-Associated Antigen-1 Membrane Glycoproteins Thrombospondins Vitronectin Fibrinogen Hydrogen Peroxide Tetradecanoylphorbol Acetate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Nathan C
Department of Medicine, Cornell University Medical College, New York 10021.
Srimal S
Farber C
Sanchez E
Kabbash L
Asch A
Gailit J
Wright S D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.3.1341
Published
1989-09-00
Pages
1341-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115779
Subset
IM
Grants
NCI NIH HHS · CA-45218 · United States
NCI NIH HHS · CA42507 · United States
NCI NIH HHS · CA43610 · United States
External Links
PubMed source DOI: 10.1083/jcb.109.3.1341 PMC Full Text View journal details
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