Abstract
We recently developed rat fibroblast cell lines that stably overproduce high levels of the beta 1 form of protein kinase C (PKC). These cells display several disorders in growth control and form small microscopic colonies in agar. In the present study we demonstrate that one of these cell lines, R6-PKC3, is extremely susceptible to transformation by an activated human bladder cancer c-H-ras oncogene (T24). Compared with control cell line R6-C1, T24-transfected R6-PKC3 cells yielded a 10-fold increase in the formation of large colonies in agar. Cell lines established from these colonies displayed a highly transformed morphology, expressed the T24-encoded p21 ras protein, continued to express high levels of PKC, and were highly tumorigenic in nude mice. These results provide genetic evidence that PKC mediates some of the effects of the c-H-ras oncogene on cell transformation. Data are also presented suggesting that optimum synergistic effects between c-H-ras and PKC require critical levels of their respective activities. These findings may be relevant to the process of multistage carcinogenesis in tissues containing cells with an activated c-H-ras oncogene.
MeSH Terms
Animals
Carcinogenicity Tests
Cell Line
Cell Transformation, Neoplastic
DNA/genetics
Genes, ras
Immunoblotting
Mice
Mice, Nude
Oncogene Protein p21(ras)
Oncogene Proteins, Viral/biosynthesis
Protein Kinase C/biosynthesis
RNA/genetics
Rats
Chemicals
Oncogene Proteins, Viral
RNA
DNA
Protein Kinase C
Oncogene Protein p21(ras)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsiao W L
Comprehensive Cancer Center, Columbia University, New York, New York 10032.
Housey G M
Johnson M D
Weinstein I B
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