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PMID: 24642476 Published · ppublish English Journal Article

Characterization of the 17 strains of regulatory T cell-inducing human-derived Clostridia.

Gut microbes ·Vol. 5 ·No. 3 ·2014-00-00 ·Pages 333-9

Narushima S, Sugiura Y, Oshima K, Atarashi K, Hattori M, Suematsu M, Honda K

Abstract

The gut microbiota plays important roles in the development of the host immune system. We have previously shown that a combination of 46 strains of commensal Clostridia isolated from conventionally reared mice can induce the accumulation of CD4(+)Foxp3(+) regulatory T (Treg) cells in the mouse colonic lamina propria. Subsequently, we succeeded in isolating and selecting 17 strains of Clostridia from a healthy human fecal sample that can significantly increase the number and function of colonic Treg cells in colonized rodents, thereby attenuating symptoms of experimental allergic diarrhea and colitis. Here we characterize each of the 17 strains of human-derived Clostridia in terms of sensitivity to antibiotics and ability to produce short chain fatty acids and other metabolites, and discuss their potential as biotherapeutics to correct dysbiosis and treat immune-inflammatory diseases.

Keywords
Clostridia Treg gut microbiota
MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Clostridium/drug effects,immunology,isolation & purification,physiology Colon/microbiology Cytosol/chemistry Dysbiosis/therapy Fatty Acids/analysis Fatty Acids, Volatile/metabolism Feces/microbiology Healthy Volunteers Humans Mice Microbial Sensitivity Tests Probiotics/administration & dosage Symbiosis T-Lymphocytes, Regulatory/immunology
Chemicals
Anti-Bacterial Agents Fatty Acids Fatty Acids, Volatile
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Narushima Seiko
RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan.
Sugiura Yuki
Department of Biochemistry; School of Medicine; Keio University; Tokyo, Japan; Japan Science and Technology Agency; Precursory Research for Embryonic Science and Technology (PRESTO); Kawaguchi, Japan.
Oshima Kenshiro
Graduate School of Frontier Sciences; The University of Tokyo; Kashiwa, Japan; Japan Science and Technology Agency; Core Research for Evolutional Science and Technology (CREST); Kawaguchi, Japan.
Atarashi Koji
RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan; Japan Science and Technology Agency; Precursory Research for Embryonic Science and Technology (PRESTO); Kawaguchi, Japan.
Hattori Masahira
Graduate School of Frontier Sciences; The University of Tokyo; Kashiwa, Japan.
Suematsu Makoto
Department of Biochemistry; School of Medicine; Keio University; Tokyo, Japan; Japan Science and Technology Agency; Exploratory Research for Advanced Technology (ERATO); Suematsu Gas Biology Project; Tokyo, Japan.
Honda Kenya
RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan; Japan Science and Technology Agency; Core Research for Evolutional Science and Technology (CREST); Kawaguchi, Japan.
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Article Info
Journal
Gut microbes
Abbr.
Gut Microbes
ISSN
1949-0984
DOI
10.4161/gmic.28572
Published
2014-00-00
Epub
2014-00-18
Pages
333-9
Language
English
Region
United States
NLM ID
101495343
PMCID
PMC4153770
Subset
IM
External Links
PubMed source DOI: 10.4161/gmic.28572 PMC Full Text View journal details
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