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PMID: 17699621 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 34 ·2007-08-21 ·Pages 13780-5

Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR

Abstract

The two primary human inflammatory bowel diseases, Crohn's disease (CD) and ulcerative colitis (UC), are idiopathic relapsing disorders characterized by chronic inflammation of the intestinal tract. Although several lines of reasoning suggest that gastrointestinal (GI) microbes influence inflammatory bowel disease (IBD) pathogenesis, the types of microbes involved have not been adequately described. Here we report the results of a culture-independent rRNA sequence analysis of GI tissue samples obtained from CD and UC patients, as well as non-IBD controls. Specimens were obtained through surgery from a variety of intestinal sites and included both pathologically normal and abnormal states. Our results provide comprehensive molecular-based analysis of the microbiota of the human small intestine. Comparison of clone libraries reveals statistically significant differences between the microbiotas of CD and UC patients and those of non-IBD controls. Significantly, our results indicate that a subset of CD and UC samples contained abnormal GI microbiotas, characterized by depletion of commensal bacteria, notably members of the phyla Firmicutes and Bacteroidetes. Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances.

MeSH Terms
Humans Inflammatory Bowel Diseases/classification,genetics,microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal/genetics
Chemicals
RNA, Ribosomal
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Frank Daniel N
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
St Amand Allison L
Feldman Robert A
Boedeker Edgar C
Harpaz Noam
Pace Norman R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0706625104
Published
2007-08-21
Epub
2007-00-15
Pages
13780-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1959459
Subset
IM
Grants
NIAID NIH HHS · N01AI30055 · United States
Databases
GENBANK
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PubMed source DOI: 10.1073/pnas.0706625104 PMC Full Text View journal details
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