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PMID: 17021222 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Culture-independent analysis of indomethacin-induced alterations in the rat gastrointestinal microbiota.

Applied and environmental microbiology ·Vol. 72 ·No. 10 ·2006-10-00 ·Pages 6707-15

Dalby AB, Frank DN, St Amand AL, Bendele AM, Pace NR

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of approximately 1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quantitative PCR results from rats treated with indomethacin and untreated rats revealed that organisms closely related to Enterococcus faecalis were heavily enriched in the small intestine and mesenteric lymph nodes of the treated rats. These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances.

MeSH Terms
Animals Anti-Inflammatory Agents, Non-Steroidal/adverse effects Ecosystem Enterococcus faecalis/drug effects,physiology Female Gastrointestinal Diseases/chemically induced Indomethacin/adverse effects Intestine, Small/drug effects,microbiology Molecular Sequence Data Peptic Ulcer/chemically induced Rats Rats, Inbred Lew
Chemicals
Anti-Inflammatory Agents, Non-Steroidal Indomethacin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dalby Andrew B
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Frank Daniel N
St Amand Allison L
Bendele Alison M
Pace Norman R
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.00378-06
Published
2006-10-00
Pages
6707-15
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1610281
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1128/AEM.00378-06 PMC Full Text View journal details
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