Home LiteratureArticle Details
PMID: 11823207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited.

Applied and environmental microbiology ·Vol. 68 ·No. 2 ·2002-02-00 ·Pages 673-90

Leser TD, Amenuvor JZ, Jensen TK, Lindecrona RH, Boye M, Møller K

Abstract

The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a <97% sequence similarity to any sequences in the database and may represent yet-uncharacterized bacterial genera or species. The phylotypes were affiliated with 13 major phylogenetic lineages. Three hundred four phylotypes (81%) belonged to the low-G+C gram-positive division, and 42 phylotypes (11.2%) were affiliated with the Bacteroides and Prevotella group. Four clusters of phylotypes branching off deeply within the low-G+C gram-positive bacteria and one in the Mycoplasma without any cultured representatives were found. The coverage of all the samples was 97.2%. The relative abundance of the clones approximated a lognormal distribution; however, the phylotypes detected and their abundance varied between two libraries from the same sample. The results document that the intestinal microbial community is very complex and that the majority of the bacterial species colonizing the gastrointestinal tract in pigs have not been characterized.

MeSH Terms
Animals Bacteria/classification,genetics,isolation & purification Culture Media DNA, Bacterial/analysis DNA, Ribosomal/analysis Digestive System/microbiology Ecosystem Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Swine/microbiology
Chemicals
Culture Media DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Leser Thomas D
Department of Microbiology, Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. tl@svs.dk
Amenuvor Joanna Z
Jensen Tim K
Lindecrona Rikke H
Boye Mette
Møller Kristian
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.2.673-690.2002
Email
tl@svs.dk
Published
2002-02-00
Pages
673-90
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC126712
Subset
IM
Databases
GENBANK
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PubMed source DOI: 10.1128/AEM.68.2.673-690.2002 PMC Full Text View journal details
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